Protein Info for ABIE53_003364 in Paraburkholderia graminis OAS925

Annotation: tRNA threonylcarbamoyladenosine biosynthesis protein TsaE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02367: TsaE
  • amino acids 24 to 161 (138 residues), 116.5 bits, E=3.9e-38
• TIGR00150: tRNA threonylcarbamoyl adenosine modification protein YjeE
  • amino acids 25 to 172 (148 residues), 111.4 bits, E=1.5e-36

Best Hits

Swiss-Prot: 47% identical to TSAE_ECO57: tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (tsaE) from Escherichia coli O157:H7

KEGG orthology group: K06925, UPF0079 ATP-binding protein (inferred from 92% identity to bug:BC1001_2864)

MetaCyc: 47% identical to N6-L-threonylcarbamoyladenine synthase, TsaE subunit (Escherichia coli K-12 substr. MG1655)
RXN-14570 [EC: 2.3.1.234]

Predicted SEED Role

"TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA"

MetaCyc Pathways

• N⁶-L-threonylcarbamoyladenosine³⁷-modified tRNA biosynthesis (1/2 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.234

Protein Sequence (187 amino acids)

>ABIE53_003364 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (Paraburkholderia graminis OAS925)
MPDHPDRAIAPPAEVLLERVFSFADEAATLAFGERFARAIESVAVAHDGNANAQAFHGLQ
VQLVGDLGAGKTTLVRATLRGLGHTGRVRSPTYTLVEPYVLERPTGELALYHFDLYRFTD
PAEWADAGFREYFDSGAVCLVEWPQRAGALLGVPDLVFSLDLAGEGDGRTLVAQAYSETG
KACLERC