Protein Info for ABIE53_002773 in Paraburkholderia graminis OAS925

Annotation: lysyl-tRNA synthetase class 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 508 TIGR00499: lysine--tRNA ligase" amino acids 15 to 506 (492 residues), 657.6 bits, see alignment E=6e-202 PF01336: tRNA_anti-codon" amino acids 68 to 145 (78 residues), 56.9 bits, see alignment E=1.7e-19 PF00152: tRNA-synt_2" amino acids 161 to 504 (344 residues), 320.2 bits, see alignment E=1.4e-99

Best Hits

Swiss-Prot: 98% identical to SYK_PARXL: Lysine--tRNA ligase (lysS) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 98% identity to bgf:BC1003_1174)

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (508 amino acids)

>ABIE53_002773 lysyl-tRNA synthetase class 2 (Paraburkholderia graminis OAS925)
MNEPIQPNLVPEVDANKIIAERREKLRELREQGVAYPNDFRPTHHAEDLQAQFEHTDKEA
LEANPLEVAIAGRMMLKRVMGKASFATVRDGSGQIQFFITPADVGQETYDAFKKWDMGDI
VAARGVLFRTNKGELSVRCTELRLLSKSLRPLPDKFHGLSDQEMKYRQRYVDLIVTPETR
KTFVARTRAISSIRKFMADAGFMEVETPMLHPIPGGAAAKPFTTHHNALDMQMFLRIAPE
LYLKRLVVGGFERVFEVNRNFRNEGVSVRHNPEFTMIEFYAAYTDYKWVMDFTEQLIRQA
AIDALGTATITYQGRELDLAKPFHRLTITQAIQKYAPQYTNEQLADSAFLRTELKKFGVD
ASQPQFLNAGVGSLQLALFEETAESQLWEPTYIIDYPLEVSPLARASDKVEGITERFELF
ITGREIANGFSELNDPEDQAVRFRKQVDQKDAGDEEAMFYDADYIRALEYGMPPAGGCGI
GIDRLVMMLTDSPSIRDVILFPHLRRED