Protein Info for ABIE53_002452 in Paraburkholderia graminis OAS925

Annotation: 2-oxoglutarate dehydrogenase E2 component (dihydrolipoamide succinyltransferase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 TIGR01347: dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex" amino acids 4 to 426 (423 residues), 560.3 bits, see alignment E=1.4e-172 PF00364: Biotin_lipoyl" amino acids 5 to 77 (73 residues), 74.7 bits, see alignment E=6.4e-25 PF02817: E3_binding" amino acids 122 to 155 (34 residues), 42.9 bits, see alignment 7e-15 PF00198: 2-oxoacid_dh" amino acids 197 to 425 (229 residues), 282.3 bits, see alignment E=4.3e-88

Best Hits

Swiss-Prot: 82% identical to ODO2_CUPNH: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (sucB) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00658, 2-oxoglutarate dehydrogenase E2 component (dihydrolipoamide succinyltransferase) [EC: 2.3.1.61] (inferred from 97% identity to bpy:Bphyt_1641)

Predicted SEED Role

"Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61)" in subsystem TCA Cycle (EC 2.3.1.61)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (427 amino acids)

>ABIE53_002452 2-oxoglutarate dehydrogenase E2 component (dihydrolipoamide succinyltransferase) (Paraburkholderia graminis OAS925)
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLAQV
IANDGDTVTADQVIAKIDTEGKAGAAAVEAEVKPAPEASPAPAPAAAPAAQAASATGANT
AASPAAGKLMAEKGLSSGDVAGTGRDGRITKGDVLTAGAPAAKAAAPAPAAAPKAAKPSL
PDVKAPASADQWLKDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMDLRN
KYKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRG
LVVPILRNADQLSLADIEKKIAEFGQKAKDGKLSIDEMTGGTFSISNGGVFGSMLSTPII
NPPQSAILGVHATKERAVVENGQIVIRPMNYLALSYDHRIIDGREAVLSLVAMKDALEDP
ARLLLDL