Protein Info for ABIE53_002068 in Paraburkholderia graminis OAS925

Annotation: ribosomal protein S12 methylthiotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 16 to 461 (446 residues), 327.5 bits, see alignment E=1.2e-101 PF00919: UPF0004" amino acids 16 to 95 (80 residues), 68.2 bits, see alignment E=7.8e-23 TIGR01125: ribosomal protein S12 methylthiotransferase RimO" amino acids 16 to 461 (446 residues), 556.2 bits, see alignment E=6e-171 PF04055: Radical_SAM" amino acids 156 to 342 (187 residues), 81.3 bits, see alignment E=1.4e-26 PF18693: TRAM_2" amino acids 398 to 463 (66 residues), 78.7 bits, see alignment E=4.4e-26

Best Hits

Swiss-Prot: 98% identical to RIMO_PARPJ: Ribosomal protein S12 methylthiotransferase RimO (rimO) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K14441, ribosomal protein S12 methylthiotransferase [EC: 2.-.-.-] (inferred from 97% identity to bgf:BC1003_1560)

MetaCyc: 66% identical to ribosomal protein S12 methylthiotransferase RimO (Escherichia coli K-12 substr. MG1655)
RXN0-6366 [EC: 2.8.4.4]

Predicted SEED Role

"Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.- or 2.8.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (463 amino acids)

>ABIE53_002068 ribosomal protein S12 methylthiotransferase (Paraburkholderia graminis OAS925)
MSQTTSSASPTPSSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTC
GFIDEAVQESLDAIGEALNENGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHAVGE
VMQHVHMHLPKPHDPFVDLVPPAGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSR
PVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVGALGEL
AAQYGAWVRLHYVYPYPSVDEVIPMMATGPYKGHVLPYLDVPFQHAHPEVLKRMKRPANA
EKVMERVKAWREMCPDLTIRSTFIAGFPGETEEQFQALLDFIREAELDRVGCFAYSPVEG
ATANELDGALPDEVREERRARFMEVAEQVSAKRIAKKVGKTLKVLVDEINADGGIGRTAA
DAPEIDGVVYIAPAAKASKRYKVGDFVSVKITGADGHDLWGEV