Protein Info for ABIE53_001664 in Paraburkholderia graminis OAS925

Annotation: acetyl-CoA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 45 to 675 (631 residues), 971.1 bits, E=1.1e-296
• PF16177: ACAS_N
  • amino acids 50 to 104 (55 residues), 77.2 bits, 9.9e-26
• PF00501: AMP-binding
  • amino acids 112 to 498 (387 residues), 300.3 bits, E=2.9e-93
• PF13193: AMP-binding_C
  • amino acids 562 to 643 (82 residues), 96.2 bits, E=2.8e-31

Best Hits

Swiss-Prot: 92% identical to ACSA_PARP8: Acetyl-coenzyme A synthetase (acsA) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 96% identity to bpy:Bphyt_1416)

MetaCyc: 79% identical to acetyl-CoA synthetase (Cupriavidus necator)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• β-alanine biosynthesis II (5/6 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• acrylate degradation II (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• chitin deacetylation (2/4 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (4/9 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (2/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1, 6.2.1.17

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17

Protein Sequence (678 amino acids)

>ABIE53_001664 acetyl-CoA synthetase (Paraburkholderia graminis OAS925)
MAHERAPAQVFIFTKGLSMSAIESVLQERRVFPPSAQAAAGATISGMDAYHALTAEAERD
YEGFWGRLARETLSWNKPFTKVLDESNAPFYTWFEDGQLNASYNSIDRHVEAGNGERVAI
VFEADDGTVTNVTYQDLLQRVSRFANALKKRGVKKGDRVVIYMPMSIEGIVAMQACARIG
ATHSVVFGGFSSKSLNERLVDVGAVALVTSDEQMRGGKALPLKNIADEALAMGGCDAVKS
VIVYKRTGGKIAWDEARDLWMHELMQAESDQCAPEWVGAEHPLFILYTSGSTGKPKGVQH
STGGYLLWAAQTMKWTFDWKANDVFWCTADIGWITGHSYITYGPLTLGGTQVVFEGVPTY
PNAGRFWDMIAKHKVTLFYTAPTAIRSLIKAAEADQKVHPKSYDLSTLRIIGTVGEPINP
EAWVWYHENVGGGRCPIVDTWWQTETGGHMITPLPGATPLVPGSCTLPLPGIMAAVVDET
GQDVPNGQGGILVVKRPWPSMLRNVWGDPDRYKKSYFPEELGGRLYLAGDGAVRDKETGY
FTIMGRIDDVLNVSGHRLGTMEIESALVSNPIVAEAAVVGRPDATTGEAVCAFVVLKRAR
PEGEEAVKLANELRNWVGKEIGPIAKPKDIRFGENLPKTRSGKIMRRLLRSLAKGEAITQ
DVSTLENPAILDQLGESL