Protein Info for ABIE53_000833 in Paraburkholderia graminis OAS925

Annotation: ATP-dependent DNA helicase RecG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 746 PF17191: RecG_wedge" amino acids 67 to 213 (147 residues), 40 bits, see alignment E=7.6e-14 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 77 to 718 (642 residues), 713.4 bits, see alignment E=1.6e-218 PF04851: ResIII" amino acids 336 to 484 (149 residues), 33.9 bits, see alignment E=7.7e-12 PF00270: DEAD" amino acids 342 to 490 (149 residues), 86.3 bits, see alignment E=5.3e-28 PF00271: Helicase_C" amino acids 536 to 640 (105 residues), 58.6 bits, see alignment E=1.8e-19 PF19833: RecG_dom3_C" amino acids 669 to 732 (64 residues), 41.9 bits, see alignment E=2.3e-14

Best Hits

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 93% identity to bgf:BC1003_0436)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (746 amino acids)

>ABIE53_000833 ATP-dependent DNA helicase RecG (Paraburkholderia graminis OAS925)
MPLSDRRLPSATDDASAEPAPGVARGGAAAQGKRAARTGSAKAAEKSAAKSAARPAAKAA
DKPVDKLAKLGLTRDIDLVLHLPMRYEDETSLTPIGHLLPGGIAQTEGVVFDNEIAYRPR
RQLLVKLRDDAGDELVLRFLNFYGSQVKQMAIGARLRVRGDVRGGFFGMEMVHPAVRVVD
EDTPLPQALTPVYPSTAGVTQAYLRKSIDNALSRTSLPELLPEAVARQWLQPLGVPSLMD
AVRTLHHPDAQSDETALIDGTHPAWVRIKFEELLAQQMSLKRAHDERRTRAAPAMPRRKL
GDEAALVARLLKALPFSLTKAQERVGGEIALDLTQPHPMQRLLQGDVGSGKTIVAALAAA
QAIDAGYQAALMAPTEILAEQHARKLRGWLEPLGVSVAWLAGSLKAKEKRAAIEAAALGT
AQLVIGTHAIIQDTVEFARLGLVIVDEQHRFGVAQRLALRAKAQNAADGARDFQPHQLMM
SATPIPRTLAMTYYADLDVSTIDELPPGRTPILTKLVSDARREEVIGRVREAALTGRQVY
WVCPLIEESETLQLQTAVETYETLVAALPELKVGLVHGRLAPAEKAAVMDAFTRNEVQLL
VATTVIEVGVDVPNASLMVIEHAERFGLAQLHQLRGRVGRGSAASVCVLLYTGPLSMTAR
ARLQTMRETTDGFEIARRDLEIRGPGEFLGARQSGAAMLRFADLQNDQWLIEPAREAAAA
LLEQYPEVVAQHLARWLGAREQYLKA