Protein Info for ABIE53_000692 in Paraburkholderia graminis OAS925

Annotation: phosphoenolpyruvate-protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 644 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00381: PTS-HPr" amino acids 6 to 45 (40 residues), 26.3 bits, see alignment 1.3e-09 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 83 to 635 (553 residues), 459.7 bits, see alignment E=6.7e-142 PF05524: PEP-utilisers_N" amino acids 85 to 204 (120 residues), 74.6 bits, see alignment E=1.5e-24 PF00391: PEP-utilizers" amino acids 229 to 302 (74 residues), 71 bits, see alignment E=1.1e-23 PF02896: PEP-utilizers_C" amino acids 330 to 613 (284 residues), 338.6 bits, see alignment E=6e-105

Best Hits

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9) / PTS system, glucose-specific IIA component" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (644 amino acids)

>ABIE53_000692 phosphoenolpyruvate-protein phosphotransferase (Paraburkholderia graminis OAS925)
MRYEGRKAAIESVVGLLGLGAGQGATVELLGVGPQAKAAVDAIAHELTREAHGEVEEKPA
RQSSQAPQTAAPAAGEALAPNTLAGVCASPGVAVGKLVRWDDADLDPPEQASGTSAAESR
LLDKAIATVDADLGTTVRDASQRGAVGEAGIFAVHRVLLEDPTLLDAARDLISLGKSAGF
AWREAIRAQIGILNNVEDALLAERAADLRDIEKRVLRALGYTNAAARTLPDEAVLAADEF
TPSDLSSLDRSRVTALVMARGGATSHAAILARQAGIPAMVAVGDALHAIPEGAQVVVNAT
NGRLEFAPTELDVERARLERNRLAGVREANRRMSQQAAVTADGRAIEVAANIATLDDAKT
AVENGADSVGLLRTELLFIHRAAAPTTDEHRQSYQGIADALGGRTAIIRTLDVGADKEVD
YLTLPPEPNPALGLRGIRLAQVRPDLLDDQLRGLLAVKPFGAVRILLPMVTDVGELIRIR
KRIDEFARELGRTEPIEVGVMVEVPSAALLADQLAQHADFLSIGTNDLTQYTLAMDRCQA
DLAAQADGLHPAVLRLIAATVQGANKHGKWVGVCGALAGDPLAMPLLVGLGVTELSVDPV
SVPGIKARVRNLDYQLCRQRAQDALALESAQAVRAVSRETWPLD