Protein Info for ABIE53_000340 in Paraburkholderia graminis OAS925

Annotation: DNA gyrase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 823 TIGR01059: DNA gyrase, B subunit" amino acids 13 to 823 (811 residues), 968.8 bits, see alignment E=6.7e-296 PF02518: HATPase_c" amino acids 40 to 182 (143 residues), 51.5 bits, see alignment E=3.3e-17 PF00204: DNA_gyraseB" amino acids 244 to 413 (170 residues), 195.1 bits, see alignment E=1.7e-61 PF01751: Toprim" amino acids 442 to 554 (113 residues), 67.4 bits, see alignment E=2.6e-22 PF18053: GyrB_insert" amino acids 588 to 749 (162 residues), 166.4 bits, see alignment E=1.4e-52 PF00986: DNA_gyraseB_C" amino acids 751 to 812 (62 residues), 100.9 bits, see alignment 8.8e-33

Best Hits

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 89% identity to bmn:BMA10247_0003)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (823 amino acids)

>ABIE53_000340 DNA gyrase subunit B (Paraburkholderia graminis OAS925)
MTETNNTQPDNSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEAL
AGYCNDIQVIIHADNSISITDNGRGVPTGLKMDDKHEPKRSAAEIVMTELHAGGKFDQNS
YKVSGGLHGVGVSCVNALSAWLRLTIRRDGKKHFMEFHRGVPQNRVIEEIDGVAVSPIQV
VGDTENRGTEVHFMADETIFGNVEYHYDILAKRIRELSFLNNGVRIRLTDQRTGKEDDFA
FVGGVKGFVEYINKTKSVLHPTIFHVSGEKDGVGVEVAMQWNDSYNENVLCFTNNIPQRD
GGTHLTGLRAAMTRVLNKYIADHEVAKKAKVETSGDDMREGLSCVLSVKVPEPKFSAQTK
DKLVSSEVRAPVEDVVAKALEEFLLETPNDAKIICGKIVDAARARDAARKAREMTRRKGV
LDGVGLPGKLADCQEKDPAKSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKVLNVEKAR
YDKLLSSEQIVTLITALGCGIGKEDYNLDKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQ
MPEMIERGYIYIAQPPLFKIKAGKDERYLKDESEVNAHILKLALQGSELLASENATPITG
DALGELARAYLLAQAVVNRLSRLYDAGALEAVMDGVVIDLSSEAAAETSAKALEAKLRDD
ALKPEVRVTTMYDPVRELRSLKVARTHHGNQKISVFDEEFQLTADYQQLVNTANTFKGLI
GAGAVIKRGERSMAVTDFKSAMKWLLADAERNISKQRYKGLGEMNPGQLWETTMDPTVRR
LLRVQIEDAIAADGIFTTLMGDDVEPRRAFIESNALRAGNIDV