Protein Info for ABIE53_000313 in Paraburkholderia graminis OAS925

Annotation: glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 548 PF04262: Glu_cys_ligase" amino acids 28 to 401 (374 residues), 534.6 bits, see alignment E=5e-165 TIGR01434: glutamate--cysteine ligase" amino acids 37 to 538 (502 residues), 629.8 bits, see alignment E=1.6e-193

Best Hits

Swiss-Prot: 61% identical to GSH1_PSEA7: Glutamate--cysteine ligase (gshA) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 95% identity to bgf:BC1003_3505)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.2

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (548 amino acids)

>ABIE53_000313 glutamate--cysteine ligase (Paraburkholderia graminis OAS925)
MAADNARLASMPNTTSSRTTDAFLHRLSVLTSGPQRDALLRGLRGIERESLRVTHEGQLA
LTPHPRALGSALTHPSLTTDYSEALIELITPAEHDVALTLEKLDTLHRFVYANLGDEILW
NNSMPGLLPDTDEGIPIADYGSSNIGKLKYVYRIGLALRYGRTMQCIAGIHYNYSLNEEV
WRLLHAEQQSTANAVDFQSDRYLALIRNFRRTNWLLMYLFGASPALDRRFLRERKHSLET
FDADTLYRPYATSLRMSDLGYSNTTAQAALHADYDTLPGYLDALAKAVSQPYPAYEAIGT
QRDGEWVQINTNVLQIENEFYSTIRPKRVTYPGERPLHALAARGVQYVEVRCMDIDPFEP
VGISLETSRFLDAYLLVCALDDSAPLPPPAYCEANQNFARVTTEGRKPGLELTRDGQTIA
MTEWADELFVKIEAAAAALDALNGGDAHARSVAVQRAKLADASLTPSARVLQTMREKQQS
FLEFGLEQSEAHAAHFRARPLPADVAKEFEELATQSLEEQAKLEREEVGSFDAFVAAYRA
YTLNRFSV