Protein Info for ABIE53_000246 in Paraburkholderia graminis OAS925

Annotation: primosomal protein N

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 749 PF17764: PriA_3primeBD" amino acids 8 to 103 (96 residues), 74.4 bits, see alignment E=1.6e-24 PF04851: ResIII" amino acids 207 to 373 (167 residues), 48.4 bits, see alignment E=3.2e-16 PF00270: DEAD" amino acids 210 to 374 (165 residues), 61.7 bits, see alignment E=2.3e-20 TIGR00595: primosomal protein N'" amino acids 229 to 747 (519 residues), 554.5 bits, see alignment E=1.1e-170 PF18319: PriA_CRR" amino acids 465 to 491 (27 residues), 37.3 bits, see alignment (E = 6.2e-13) PF00271: Helicase_C" amino acids 513 to 609 (97 residues), 32.4 bits, see alignment E=2.9e-11 PF18074: PriA_C" amino acids 652 to 746 (95 residues), 55.7 bits, see alignment E=2.2e-18

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 91% identity to bpy:Bphyt_3889)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (749 amino acids)

>ABIE53_000246 primosomal protein N (Paraburkholderia graminis OAS925)
MTTAVFVRVALDHPLPTLFDYRWNMSTAAVPGTLVSVPFGKRDAVGLVCEVTAHSEVPAD
RLRAVNGACTACPPLSAEWLRLAAFAADYYQRGLGEVALPALPQALRDASRWSRLLAPEE
RYSLTPQGHLALADALPARATALRSLAQALAANDFLSAADARALHPKAIATLAAWQAEGW
VALELVDAATVLPALTSTATPPPTLPTLTDEQAAAVEAIRDARGFAPFLLHGVTGSGKTE
VYLRALAEILAANADAQALVLVPEINLTPQFEAAFRARFAALADTAIVTLHSGLAEGERA
RNWFAAHTGRARIVLGTRLAVLASLPRLAVIVVDEEHDPAYKQQEGLRYSARDLAIYRAK
QLGLPVVLGSATPSLESWWQADQGRYKRLTLSRRAVADAVLPTVRLIDLEEERRRGRASV
EGLSGPLIAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHKPERA
LRCHHCGWESRIPRSCPECGNVDIEPMGRGTQRVEETLASAVPGARVLRIDADSTRRKGS
AQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQ
VSGRAGRAGLPGEVLVQTRYPRHALYHALGRHDYVGFANSTLAERRDAHLPPFVYQALLR
AEGRTLEAALAFLQQAAAELAGIPAAERVTVYDAVPLTIVKVMHVHRAQLLVESASRAAL
QATLRAWQPLLRGLKGVLRWSLEVDPLDI