Protein Info for ABIE53_000236 in Paraburkholderia graminis OAS925

Annotation: aminomethyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 372 TIGR00528: glycine cleavage system T protein" amino acids 4 to 365 (362 residues), 385.8 bits, see alignment E=9.1e-120 PF01571: GCV_T" amino acids 9 to 261 (253 residues), 291.5 bits, see alignment E=5.1e-91 PF08669: GCV_T_C" amino acids 286 to 363 (78 residues), 56.4 bits, see alignment E=2.3e-19

Best Hits

Swiss-Prot: 89% identical to GCST_PARP8: Aminomethyltransferase (gcvT) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K00605, aminomethyltransferase [EC: 2.1.2.10] (inferred from 96% identity to bgf:BC1003_3424)

Predicted SEED Role

"Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 2.1.2.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.10

Use Curated BLAST to search for 2.1.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (372 amino acids)

>ABIE53_000236 aminomethyltransferase (Paraburkholderia graminis OAS925)
MTELKHTPLNATHRALNARMVDFGGWDMPVNYGSQIDEHRAVRTDAGMFDVSHMCVVDFT
GERVRAFFEYALANNVGKLQTPGRALYSCMLNPDGGVIDDLIVYYFGEDHFRVVVNAGTA
DKDIAWFNQLNEGGFGLTITPRRDLAIVAVQGPNAREKVWQTAPSLRAATEALKPFNAAR
IETAPFGELTVARTGYTGEDGFEIIVPADHVVALWNALQAQGVRPAGLGARDTLRLEAGM
NLYGQDMDDNTSPLDAGLAWTVDLASPREFVGKSRLELDGSKAAFVGLILRKENGKAAGV
LRAHQKVVTPHGEGEITSGTFSPTMQESIAFARVPQGVQPGDTVHVQIRDKSVPASVVKL
PFVRNGKVLAAA