Protein Info for ABIE51_RS17625 in Lysobacter sp. OAE881

Annotation: lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04413: Glycos_transf_N
  • amino acids 42 to 219 (178 residues), 223.4 bits, E=1.6e-70
• PF00534: Glycos_transf_1
  • amino acids 308 to 406 (99 residues), 36.6 bits, E=3.4e-13

Best Hits

KEGG orthology group: K02527, 3-deoxy-D-manno-octulosonic-acid transferase [EC: 2.-.-.-] (inferred from 68% identity to xcc:XCC3337)

MetaCyc: 41% identical to lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (Vibrio cholerae O1 biovar El Tor str. N16961)
RXN2G6Z-2 [EC: 2.4.99.12]

Predicted SEED Role

"Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (EC 2.4.99.12) [often with (EC 2.4.99.13) also]" (EC 2.4.99.12, EC 2.4.99.13)

MetaCyc Pathways

• superpathway of (Kdo)₂-lipid A biosynthesis (15/17 steps found)
• Kdo transfer to lipid IV_A (Brucella) (2/2 steps found)
• Kdo transfer to lipid IV_A (E. coli) (2/2 steps found)
• Kdo transfer to lipid IV_A (H. pylori) (2/2 steps found)
• Kdo transfer to lipid IV_A (P. gingivalis) (2/2 steps found)
• Kdo transfer to lipid IV_A (P. putida) (2/2 steps found)
• Kdo transfer to lipid IV_A (generic) (2/2 steps found)
• Kdo transfer to lipid IV_A (Haemophilus) (1/2 steps found)
• Kdo transfer to lipid IV_A (Vibrio cholerae serogroup O1 El Tor) (1/2 steps found)
• Kdo transfer to lipid IV_A (Chlamydia) (2/5 steps found)
• superpathway of Kdo₂-lipid A biosynthesis (14/25 steps found)

KEGG Metabolic Maps

• Lipopolysaccharide biosynthesis
• Puromycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.- or 2.4.99.12 or 2.4.99.13

Protein Sequence (431 amino acids)

>ABIE51_RS17625 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase (Lysobacter sp. OAE881)
MPKDPIERLLRGLYSVALYLLAPITVYHLIWRGLRQPAYFLRWQERYAHYGDAPPMRTLW
VHAVSVGEVNAAVPLVNALRRARPDLRLLVTTITPTGSERVQALWGEAVEHVYLPYDLPG
AVGRFLRHYRPYAALIMETELWPSLLFGCRDAGIPAVILNARLSERSLRGYRVLAPLVAR
ALRTVRTVAAQSHADAQRFVRLGANPEQVAEVGNLKFDVAVPEDLAGFAAQCRSRCGERP
VWIAASTHEDEEMAVIAIHRQLRAHFPDLLMLWAPRHPERFRTVADTARAAGWNVSTRSR
QQWPQADDAVFVIDTLGELMSFYACADVAFVGGSLQAIGGHNLLEPAATGTAIVTGPHLH
NFVEIAQRLEDAGALCVAPDAEHVERALAELLSDRERRERMTDAGRALVETGRGALARTL
DLLQPLLPPQA