Protein Info for ABIE51_RS17015 in Lysobacter sp. OAE881

Annotation: periplasmic nitrate reductase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 830 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 2 to 26 (25 residues), 22 bits, see alignment (E = 1.6e-08) TIGR01706: periplasmic nitrate reductase, large subunit" amino acids 3 to 828 (826 residues), 1566.1 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 41 to 93 (53 residues), 65.8 bits, see alignment 4.1e-22 PF00384: Molybdopterin" amino acids 97 to 570 (474 residues), 251.3 bits, see alignment E=2.2e-78 PF01568: Molydop_binding" amino acids 716 to 824 (109 residues), 90.7 bits, see alignment E=9.5e-30

Best Hits

Swiss-Prot: 75% identical to NAPA_BORBR: Periplasmic nitrate reductase (napA) from Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50)

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 75% identity to bbr:BB2800)

MetaCyc: 66% identical to nitrate reductase large subunit (Aliivibrio fischeri)
Nitrate reductase (cytochrome). [EC: 1.9.6.1]

Predicted SEED Role

"Periplasmic nitrate reductase precursor (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.99.4 or 1.9.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (830 amino acids)

>ABIE51_RS17015 periplasmic nitrate reductase subunit alpha (Lysobacter sp. OAE881)
MTTRREFIRNTAVATAAAAAGLPLPGDTANVLTEGDLTALKWDKAPCRFCGTGCGVNVAV
KEGRVVATHGDVHAEVNRGINCVKGYFLSKIMYGSDRLTAPLLRKKNGVYAKDGEFAEVT
WDEAFDVMAAQFKKVLKEKGADHIGMFGSGQWTIWEGYAANKLMKAGFRSNHIDPNARHC
MASAVMGFMRTFGMDEPMGCYDDIEAADAFVLWGSNMAEMHPILWTRVTDRRLTHPHVKV
AVLSTFEHRSFELADIPIVFKPQTDLVILNYIANHIITSGKVNRQFVDRHTSFKRGNDDI
GYGLRPDNPLELKAKNAKDANGGEVIDFDQFAAFVKPYTLDKAVEMTGVERGWLEKLANL
YADPNVKVMSFWTMGFNQHTRGVWCNNMVYNIHLLTGKIATPGNSPFSLTGQPSACGTAR
EVGTFSHRLPADMLVANPEHRKHAEEIWKIPHGIINPKPGYHAVEQNRMLKDGKLNAYWV
QVNNNMQAAANLYEETWPGYRNPQNFIVVSDAYPTVTAQAADLILPAAMWVEKEGAYGNA
ERRTQFWHQLVNAPAQARSDLWQLMEFSKRFTTDECWPADILAANPQFKGKTLFDVLYKN
GNVDRYPVSDIQQGYANDESTHFGFYVQKGLFEEYATFGRGHGHDLAPFDAYHKTRGLRW
PVLEGRETRWRYREGSDVYVPPGEGFRFYGNKDGRAVIWALPYEPAAESPDAEFDLWLVT
GRVLEHWHSGSMTMRVPELFRSFPTAMVFMNPDDARKRGLKRGDDVRIVSRRGEMRTRVE
TRGRNRMPEGVVFVPWFDAGQLINKVTLDATDPISKQTDYKKCAVKILGA