Protein Info for ABIE51_RS15270 in Lysobacter sp. OAE881

Annotation: threonine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 633 PF02824: TGS" amino acids 2 to 61 (60 residues), 45.2 bits, see alignment 1.5e-15 TIGR00418: threonine--tRNA ligase" amino acids 72 to 630 (559 residues), 721.2 bits, see alignment E=5.7e-221 PF07973: tRNA_SAD" amino acids 170 to 218 (49 residues), 56.4 bits, see alignment 4.6e-19 PF00587: tRNA-synt_2b" amino acids 319 to 528 (210 residues), 161.6 bits, see alignment E=4.4e-51 PF03129: HGTP_anticodon" amino acids 540 to 629 (90 residues), 77.3 bits, see alignment E=1.7e-25

Best Hits

Swiss-Prot: 74% identical to SYT_XANCB: Threonine--tRNA ligase (thrS) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 76% identity to psu:Psesu_1115)

MetaCyc: 56% identical to threonine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Threonine--tRNA ligase. [EC: 6.1.1.3]; 6.1.1.3 [EC: 6.1.1.3]; 3.1.1.- [EC: 6.1.1.3]

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (633 amino acids)

>ABIE51_RS15270 threonine--tRNA ligase (Lysobacter sp. OAE881)
MIAITLPDGSRREFENPVSVAEVAASIGAGLAKAALAGKVDGKLVDTSYRIGNDASLEIV
TDKHPDALDVLRHSTAHLLAQAVQRLYPGAQVTIGPVIDNGFYYDFAYERPFTPEDLPAI
EAEMQKIVKESLPVARSVKSRDDAVAFFRGLGEAYKAEIIESIPANEDLSLYSQGEFTDL
CRGPHVPSTDKLRAFKLMKVAGAYWRGDSNNQMLSRIYGTSWLNDKDLKAYLTQLEEAEK
RDHRKIAKAQDLFHLQEEGPGLIFWHPKGWSIWQVVEQYMRRVYRETGYGEVRCPQILDV
SLWQKSGHWDNYKDNMFFTESEKRTYALKPMNCPGHVQVFNQGLHSYRDLPIRYGEFGAC
HRNEPSGALHGILRVRGFTQDDGHIFCTEDQIESEVRAFHEQALKVYGDFGFTDIQIKIA
LRPDSRLGDDATWDKAENALRSALSAAGVEWQELPGEGAFYGPKIEYHLQDAIGRTWQLG
TMQVDFMMPGRLGAEYVDEHSQRRHPVMLHRAIVGSMERFIGILIEHHAGQFPAWLAPVQ
AVVMNITDAQADYVEEIRKSLANQGFRVHSDLRNEKIGYKIREHTLQRVPYLLVIGDREK
ENGQIAVRTRGGEDLGTMSVADFASRLRSEGVQ