Protein Info for ABIE51_RS14525 in Lysobacter sp. OAE881

Annotation: formate-dependent phosphoribosylglycinamide formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01142: phosphoribosylglycinamide formyltransferase 2
  • amino acids 14 to 395 (382 residues), 589.8 bits, E=1e-181
• PF02222: ATP-grasp
  • amino acids 129 to 300 (172 residues), 187.6 bits, E=3e-59
• PF02786: CPSase_L_D2
  • amino acids 129 to 217 (89 residues), 29.8 bits, E=8.6e-11
• PF07478: Dala_Dala_lig_C
  • amino acids 130 to 290 (161 residues), 25.1 bits, E=2.3e-09
• PF21244: PurT_C
  • amino acids 327 to 393 (67 residues), 67.5 bits, E=1.2e-22

Best Hits

Swiss-Prot: 80% identical to PURT_STRM5: Formate-dependent phosphoribosylglycinamide formyltransferase (purT) from Stenotrophomonas maltophilia (strain R551-3)

KEGG orthology group: K08289, phosphoribosylglycinamide formyltransferase 2 [EC: 2.1.2.2] (inferred from 81% identity to psu:Psesu_2134)

MetaCyc: 70% identical to phosphoribosylglycinamide formyltransferase 2 (Escherichia coli K-12 substr. MG1655)
GARTRANSFORMYL2-RXN [EC: 6.3.1.21]; Acetate kinase. [EC: 6.3.1.21, 2.7.2.1, 2.7.2.15]

Predicted SEED Role

"Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• Bifidobacterium shunt (12/15 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• pyruvate fermentation to acetate II (2/3 steps found)
• superpathway of acetate utilization and formation (2/3 steps found)
• acetate and ATP formation from acetyl-CoA I (1/2 steps found)
• glycine degradation (reductive Stickland reaction) (1/2 steps found)
• mixed acid fermentation (11/16 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (2/4 steps found)
• superpathway of L-threonine metabolism (12/18 steps found)
• acetyl-CoA fermentation to butanoate (4/7 steps found)
• pyruvate fermentation to acetate I (1/3 steps found)
• pyruvate fermentation to acetate IV (1/3 steps found)
• pyruvate fermentation to acetate VII (1/3 steps found)
• L-threonine degradation I (3/6 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• acetylene degradation (anaerobic) (2/5 steps found)
• ethanolamine utilization (2/5 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (12/19 steps found)
• superpathway of N-acetylneuraminate degradation (14/22 steps found)
• pyruvate fermentation to acetate and lactate II (1/4 steps found)
• purine nucleobases degradation II (anaerobic) (15/24 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (4/9 steps found)
• (S)-propane-1,2-diol degradation (1/5 steps found)
• methanogenesis from acetate (1/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (3/9 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (3/9 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (2/8 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• L-lysine fermentation to acetate and butanoate (2/10 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (4/13 steps found)
• gallate degradation III (anaerobic) (2/11 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (6/17 steps found)
• superpathway of methanogenesis (1/21 steps found)
• superpathway of L-lysine degradation (6/43 steps found)

KEGG Metabolic Maps

• Nucleotide sugars metabolism
• One carbon pool by folate
• Propanoate metabolism
• Purine metabolism
• Pyruvate metabolism
• Taurine and hypotaurine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.2

Use Curated BLAST to search for 2.1.2.- or 2.1.2.2 or 2.7.2.1 or 2.7.2.15 or 6.3.1.21

Protein Sequence (399 amino acids)

>ABIE51_RS14525 formate-dependent phosphoribosylglycinamide formyltransferase (Lysobacter sp. OAE881)
MVTIGTPLSPHGFRVLLLGSGELGKEVAIELQRFGVEVIAADRYADAPAMQVAHRSHVLD
MLDGAAVRALVALERPHLIVPEIEAIHTQTLVELEREFEARGSDVRVIPTARAARLTMDR
EGIRRLAAETLGLPTSPYRFVDTLEDYRAAVAEIGVPCVVKPVMSSSGKGQSLVRSQADI
DQAWEYAQTGGRAGAGRVIVEGFIDFEYEITLLTVRHAGGTTFCAPIGHLQRDGDYRESW
QPQAMSPRALERSQEIARAITDDLGGWGVFGVELFVKGDEVWFSEVSPRPHDTGLVTLIS
QDLSEFALHARAILGLPIPAIRQYGAAASCAVLAHGHGVPVFSDVDKALEQADTQLRLFG
KPRVEGHRRVAVTLALGEDIENARQRARDAAAALTFELR