Protein Info for ABIE51_RS11640 in Lysobacter sp. OAE881

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 640 TIGR02350: chaperone protein DnaK" amino acids 3 to 602 (600 residues), 949.3 bits, see alignment E=3.8e-290 PF00012: HSP70" amino acids 4 to 602 (599 residues), 880.6 bits, see alignment E=4.5e-269 PF06723: MreB_Mbl" amino acids 125 to 377 (253 residues), 39.4 bits, see alignment E=3.5e-14

Best Hits

Swiss-Prot: 86% identical to DNAK_XYLFT: Chaperone protein DnaK (dnaK) from Xylella fastidiosa (strain Temecula1 / ATCC 700964)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 87% identity to psu:Psesu_1655)

MetaCyc: 72% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (640 amino acids)

>ABIE51_RS11640 molecular chaperone DnaK (Lysobacter sp. OAE881)
MGKIIGIDLGTTNSCVAIMEGGKPRVIENAEGDRTTPSIVAFTKDGEVLVGASAKRQAVT
NPKNTFFAVKRLIGRKFTDAEVQKDIGLVPYKITQHENGDAWVETSDGKKMAPQQISAEV
LAKMKKTAEAYLGETVTEAVITVPAYFNDSQRQATKDAGKIAGLDVKRIINEPTAAALAY
GMDKKGGDRKVAVYDLGGGTFDVSIIEIASVDGEMQVEVLSTNGDTFLGGEDFDKRVIDY
LVEEFQKDQGIDLRKDPLALQRLKDAAERAKIELSSAQQTEVNLPYVTADASGPKHLNIK
LTRAKLEALVDDLVKRTIEPCRIALNDAGLRASEISEVILVGGQTRMPKVQSAVAEFFGK
EPRKDVNPDEAVALGAAIQGGVLAGDVKDVLLLDVTPLSLGIETLGGVFTKIIEKNTTIP
TKASQVFSTAEDNQSAVTVHVLQGEREQARYNKSLARFDLTGIEPAPRGMPQVEVSFDID
ANGILHVSAKDKKTNKEQKVEIKAGSGLSEEEIAKMVSDAEAHREEDQKFHELVQARNHA
DALIHTARSAIKDNGDKVAGDVIGRTEGAIAELETAMRGDDKGQIEAKSKALEEAAQSLF
AAASAAGQPGGPDAGAGPSGGAKSDDVVDAEFTEVKDDKK