Protein Info for ABIE51_RS11615 in Lysobacter sp. OAE881

Annotation: carbamoyl-phosphate synthase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1060 (1059 residues), 1541.5 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 240.3 bits, E=6.3e-75
  • amino acids 674 to 882 (209 residues), 129.6 bits, E=5.1e-41
• PF02787: CPSase_L_D3
  • amino acids 428 to 507 (80 residues), 96.5 bits, 2.9e-31
• PF02222: ATP-grasp
  • amino acids 683 to 852 (170 residues), 39 bits, E=2.7e-13
• PF07478: Dala_Dala_lig_C
  • amino acids 702 to 851 (150 residues), 25 bits, E=5e-09
• PF02142: MGS
  • amino acids 964 to 1049 (86 residues), 80.3 bits, E=3.8e-26

Best Hits

Swiss-Prot: 69% identical to CARB_ECOL6: Carbamoyl-phosphate synthase large chain (carB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 69% identity to aaa:Acav_2592)

MetaCyc: 69% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-arginine biosynthesis II (acetyl cycle) (8/10 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-citrulline degradation (2/3 steps found)
• superpathway of arginine and polyamine biosynthesis (12/17 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)
• L-arginine biosynthesis IV (archaea) (3/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1082 amino acids)

>ABIE51_RS11615 carbamoyl-phosphate synthase large subunit (Lysobacter sp. OAE881)
MPKRTDIKTVLIIGAGPIVIGQACEFDYSGAQACKALRDEGYRVVLVNSNPATIMTDPNM
ADAVYIEPINWQTVEKIIAKEKPDALLPTMGGQTALNCALDLADNGVLEKYNVELIGASR
EAIRMAEDRELFRVAMAEIGLECPKAEVARNFEQAIEIQAKVGYPTIIRPSFTLGGTGGG
IAYNKEEFEEIAKRGLELSPVHEILVEESVLGWKEFEMEVVRDKADNCIIVCSIENFDAM
GVHTGDSITVAPAQTLTDKEYQRLRDASIAVLRKIGVDTGGSNVQFGINAKNGRVVVIEM
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELKNEITGGLTPASFEPSIDYVVTKIP
RFAFEKFPQADARLTTQMKSVGEVMAMGRTFQESLQKALRGLETGKVGLDPTGLDLGSED
DLTKLRRELKEPGPERMFHIGDAFRAGMSVEDVHALSFVDPWFLDQIEELIAAEQDVAAA
GLSSLDKTRMRELKRMGFSDARIAQLTGTDETAVRTLRRAFGVRPVYKRVDSCAAEFATT
TAYMYSTYEDECEAEPTDRNKIIVLGGGPNRIGQGIEFDYCCVHAALALREDGFETIMVN
CNPETVSTDYDTSDRLYFEPLTLEDVLEICDLEKPKGVIVQYGGQTPLKLARALEANGVP
IIGTSPDSIDLAEDRERFQKLVQDLGLAQPLNRTARNPEEAIVLASQIGYPLVVRPSYVL
GGRAMEIVHADADLHRYMRDAVKVSNDSPVLLDRFLDNAVEVDVDVIADKDGNVLVGGVM
EHIEEAGVHSGDSSCSLPPYSLSAAVQDKLREQVVALAKSLKVVGLMNTQFAITFDDEGR
ETIYLIEVNPRASRTVPFVSKATGMALAKIAARCMVGKTLAEQGAVKEIVPEYYSVKEAI
FPFAKFQGVDPILGPEMRSTGEVMGVGRNFGAAMARAQEAGGIKALPEGGKVFVSVRDPD
KKRVLPVAQDLVKRGYTLVATEGTHRFFTENGLACERINKVTEGRPHIVDLIKNGEIAYI
VNTTEGRQAINDSFSIRREALQHRVTYSTTVSGARALVNSLEYRGTGPVWSLQELHAEVQ
GA