Protein Info for ABIE51_RS09480 in Lysobacter sp. OAE881

Annotation: glutamine-hydrolyzing GMP synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00888: GMP synthase (glutamine-hydrolyzing), N-terminal domain
  • amino acids 9 to 201 (193 residues), 252.1 bits, E=2.5e-79
• PF00117: GATase
  • amino acids 10 to 195 (186 residues), 153.1 bits, E=2.1e-48
• PF07722: Peptidase_C26
  • amino acids 73 to 179 (107 residues), 38.8 bits, E=2.7e-13
• TIGR00884: GMP synthase (glutamine-hydrolyzing), C-terminal domain
  • amino acids 208 to 521 (314 residues), 494.8 bits, E=9.8e-153
• PF02540: NAD_synthase
  • amino acids 210 to 290 (81 residues), 35.4 bits, E=2e-12
• PF03054: tRNA_Me_trans
  • amino acids 225 to 290 (66 residues), 22.2 bits, E=3e-08
• PF00958: GMP_synt_C
  • amino acids 429 to 520 (92 residues), 142.6 bits, E=9.6e-46

Best Hits

Swiss-Prot: 91% identical to GUAA_XANC5: GMP synthase [glutamine-hydrolyzing] (guaA) from Xanthomonas campestris pv. vesicatoria (strain 85-10)

KEGG orthology group: K01951, GMP synthase (glutamine-hydrolysing) [EC: 6.3.5.2] (inferred from 91% identity to xac:XAC2287)

MetaCyc: 71% identical to GMP synthetase (Escherichia coli K-12 substr. MG1655)
GMP synthase (glutamine-hydrolyzing). [EC: 6.3.5.2]; 6.3.5.2 [EC: 6.3.5.2]

Predicted SEED Role

"GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)" in subsystem Purine conversions or Staphylococcal pathogenicity islands SaPI (EC 6.3.5.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of purine nucleotide salvage (12/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Glutamate metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.2

Use Curated BLAST to search for 6.3.5.2

Protein Sequence (521 amino acids)

>ABIE51_RS09480 glutamine-hydrolyzing GMP synthase (Lysobacter sp. OAE881)
MTNIHSDKILILDFGAQYTQLIARRIREIGVYCEIWAWDHDPAEIAAFGAKGIILSGGPE
STTEEGAPRAPQEVFDSNLPVLGICYGMQTLAAQLGGATEAADAREFGHAEVELVAHDAL
LGGLTDHDGEPRIDVWMSHGDHVAKAPPGWTVTAVTDRIPVAAMAREDKRWYGVQFHPEV
THTKQGQTLLRRFVADICGCKTLWTAANIIEDQIARVREQVGSDEVILGLSGGVDSSVVA
ALLHKAIGEQLTCVFVDTGLLRWNEGDQVMAMFAEHMGVKVVRVNAADRYFKALAGVEDP
EAKRKIIGNLFVEIFDEESAKLANAKWLAQGTIYPDVIESAGSKTGKAHVIKSHHNVGGL
PEDMKLRLVEPLRELFKDEVRRLGVELGLPRAMVYRHPFPGPGLGVRILGEVKREYAELL
ARADHIFIDELRKADLYDRTSQAFAVFLPVKSVGVVGDARAYEWVIALRAVETIDFMTAH
WAHLPYDFLGRVSNRIINELRGVSRVVYDISGKPPATIEWE