Protein Info for ABIE51_RS08405 in Lysobacter sp. OAE881

Annotation: tRNA pseudouridine(55) synthase TruB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 317 TIGR00431: tRNA pseudouridine(55) synthase" amino acids 12 to 220 (209 residues), 214.7 bits, see alignment E=5.9e-68 PF01509: TruB_N" amino acids 33 to 181 (149 residues), 154.5 bits, see alignment E=5.5e-49 PF16198: TruB_C_2" amino acids 182 to 243 (62 residues), 62.7 bits, see alignment E=5.6e-21 PF09142: TruB_C" amino acids 246 to 299 (54 residues), 34 bits, see alignment 3.6e-12 PF09157: TruB-C_2" amino acids 247 to 301 (55 residues), 39.5 bits, see alignment E=8.9e-14

Best Hits

Swiss-Prot: 67% identical to TRUB_XANCB: tRNA pseudouridine synthase B (truB) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K03177, tRNA pseudouridine synthase B [EC: 5.4.99.12] (inferred from 68% identity to psu:Psesu_1827)

MetaCyc: 43% identical to tRNA pseudouridine55 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11839 [EC: 5.4.99.25]

Predicted SEED Role

"tRNA pseudouridine synthase B (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (317 amino acids)

>ABIE51_RS08405 tRNA pseudouridine(55) synthase TruB (Lysobacter sp. OAE881)
MTKAKTRFRPLDGILLLDKPQGLSSNQALQQARHLFRAEKGGHTGSLDPLATGLLPLCFG
EATKIAGLLLGSAKAYETTAVLGLTTDSDDADGAPLLERPVPALDDAAIEAALAPLRGPI
RQRAPIYSALKQGGEPLYAKARRGEAIEAPERDVVVHRFELIERDGPRLRLHVECGSGTY
VRSLVRDLGEALGCGAHVATLRRLWVDPFHAPRMITLDALRAMRESDGEAAIEDLLLPIE
AGLSQFPRVDVDASAARRLGQGQGVDCAQAADGLVAIFGPEGRCLGLGQRTGTRLAPQRL
FRWAAQSGAEQAASSAV