Protein Info for ABIE51_RS08245 in Lysobacter sp. OAE881

Annotation: tRNA pseudouridine(38-40) synthase TruA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00071: tRNA pseudouridine(38-40) synthase
  • amino acids 16 to 260 (245 residues), 198.1 bits, E=8.5e-63
• PF01416: PseudoU_synth_1
  • amino acids 23 to 126 (104 residues), 34.5 bits, E=1.2e-12
  • amino acids 165 to 267 (103 residues), 100.8 bits, E=3e-33

Best Hits

Swiss-Prot: 72% identical to TRUA_XANC5: tRNA pseudouridine synthase A (truA) from Xanthomonas campestris pv. vesicatoria (strain 85-10)

KEGG orthology group: K06173, tRNA pseudouridine synthase A [EC: 5.4.99.12] (inferred from 72% identity to xcv:XCV2872)

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Protein Sequence (278 amino acids)

>ABIE51_RS08245 tRNA pseudouridine(38-40) synthase TruA (Lysobacter sp. OAE881)
MNTPAESSPPDTAPTRRFALGIEYDGNGFCGWQRLSKHGEPEREGEETLQAALEFAISFV
AGHPVETICAGRTDAGVHAECQVVHFDSHAVRDPRSWMLGVTSRLPPSIAVRWCRPVPND
FHARFSARARRYRYRILNRPVRAALGRQYLSWERRPLDADAMHRAAQALLGENDFSSFRT
VHCQSPHAWRELQDIRVTRAGDVVEVEVQANAFLHHMVRNIVGSLLMVGSGEKPERWIAE
LLETRDRTVAGPTAPPTGLVFLGPRYPAQWNLPAEVTL