Protein Info for ABIE51_RS07020 in Lysobacter sp. OAE881

Annotation: DNA topoisomerase IV subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747 TIGR01062: DNA topoisomerase IV, A subunit" amino acids 13 to 742 (730 residues), 1031.6 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 36 to 472 (437 residues), 483.7 bits, see alignment E=5.4e-149 PF03989: DNA_gyraseA_C" amino acids 601 to 644 (44 residues), 20.4 bits, see alignment 2.7e-08 amino acids 663 to 698 (36 residues), 10.8 bits, see alignment (E = 2.8e-05)

Best Hits

Swiss-Prot: 67% identical to PARC_PSEAE: DNA topoisomerase 4 subunit A (parC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02621, topoisomerase IV subunit A [EC: 5.99.1.-] (inferred from 84% identity to psu:Psesu_0921)

Predicted SEED Role

"Topoisomerase IV subunit A (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (747 amino acids)

>ABIE51_RS07020 DNA topoisomerase IV subunit A (Lysobacter sp. OAE881)
MNDTVRPAFHGFEQIPLREYAERAYLDYSMYVVLDRALPFLGDGLKPVQRRIIYSMSELG
LNAASKPKKSARTVGDVIGKYHPHGDSACYEAMVLMAQPFSYRYPLVEGQGNFGSTDDPK
SFAAMRYTEAKLTPIAEVLLGELGQGTVDWTPNFDGTLEEPSWMPARLPHLLLNGTTGIA
VGMATDVPPHNLNEVVSACVRLLDDPDATTRDLCEHVRGPDYPTAAEIITPAADLQQMYE
TGLGSVRARAVYVKDGANLVITALPYQTSPGKVIEQIATQMRAKKLPWLEDIRDESDHAN
PTRIVLVPRSNRVDAEQLMGHLFATTDLEKSFRVNFNIIGRDGRPQVKGLKALLTEWLAF
RTETVTRRLRHRLDKVERRLHLLEGLLVAFLNLDEVIRIIRTEDEPKPVLMKRFKLSEEQ
TDYILETRLRQLARLEEMKIRGEEDELKAERERIIATLESRAKLKKLVKDELLADAKKFG
DARRSPLVARGAAQALSETELVASEPMTVVLSEKGWVRAAKGHDVDAASLSYREGDELLA
SAKSRSTQQVAFLDSTGRAYSTVVHSLPSARGNGEPLTGRFSPAPGASFQALASGDNDTR
FVLASSHGYGFVTRFENLTGRNKAGKAMLSLTPGAKVVQPAPVSSVEQDRIAVVTNVGHL
LAFPVGELPELDKGKGNKLIDIPKAKLGTERVVAVAVVAPGGTLVVKSGARTMSLSFKDL
DTYLGARATRGGLLPRGWQKVEGLGVE