Protein Info for ABIE51_RS05265 in Lysobacter sp. OAE881
Annotation: adenosylmethionine--8-amino-7-oxononanoate transaminase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 40% identical to BIOK_BACSU: L-Lysine--8-amino-7-oxononanoate transaminase (bioK) from Bacillus subtilis (strain 168)
KEGG orthology group: K00833, adenosylmethionine-8-amino-7-oxononanoate aminotransferase [EC: 2.6.1.62] (inferred from 77% identity to xal:XALc_2212)MetaCyc: 40% identical to lysine-8-amino-7-oxononanoate aminotransferase (Bacillus subtilis)
RXN-14930 [EC: 2.6.1.105]
Predicted SEED Role
"Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62)" in subsystem Biotin biosynthesis (EC 2.6.1.62)
MetaCyc Pathways
- biotin biosynthesis I (14/15 steps found)
- biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)
- biotin biosynthesis from 8-amino-7-oxononanoate II (4/4 steps found)
- biotin biosynthesis II (5/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.62
Use Curated BLAST to search for 2.6.1.105 or 2.6.1.62
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (469 amino acids)
>ABIE51_RS05265 adenosylmethionine--8-amino-7-oxononanoate transaminase (Lysobacter sp. OAE881) MDHIIARDDTAAAWRERDLAVLWHPCTQMREHLDPAGTLPLLPVERGEGAWLIGRDGRRY LDAISSWWTNLFGHAEPRIAAAIARQATTLEHVILAGVSHAPAVELAEKLLSIAPREVGR DPLAKVFYADNGSAGVEVALKMAFHWFRNRGIDGRTKFIALDNGYHGETLGALAVGDIPL YRRVYAPLLAEAMFAPSPDAYLCEEGETPAQRAHRAADALAELLERHAGEVCALILEPRV QCAGGMRMHDPAYLKRARELCDAHGVFLICDEIAVGFGRTGTLFASEQSGVQPDLLCLSK GLTGGALPLAAVLATQSIYDGFLDESREKAFLHSHSYTGNPLACAAALASMAILEQDDVI LRNRDTSQRLATLAAPFADHPHVADVRQAGMIVAFELTRGGDKRTPFDAGARIGLRAYRA ALERGVLLRPLGDVLYWMPPYCVDDDALQLLADTTRAAIDHATEEAACA