Protein Info for ABIE51_RS04925 in Lysobacter sp. OAE881

Annotation: ATP-dependent chaperone ClpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 861 TIGR03346: ATP-dependent chaperone protein ClpB" amino acids 6 to 859 (854 residues), 1473.7 bits, see alignment E=0 PF02861: Clp_N" amino acids 17 to 68 (52 residues), 43.9 bits, see alignment 1.1e-14 amino acids 95 to 145 (51 residues), 43.2 bits, see alignment 1.8e-14 PF00004: AAA" amino acids 203 to 336 (134 residues), 48.7 bits, see alignment E=5.2e-16 amino acids 602 to 720 (119 residues), 37.2 bits, see alignment E=1.9e-12 PF17871: AAA_lid_9" amino acids 342 to 444 (103 residues), 118.5 bits, see alignment E=5.8e-38 PF07724: AAA_2" amino acids 596 to 763 (168 residues), 237.2 bits, see alignment E=5e-74 PF00158: Sigma54_activat" amino acids 599 to 726 (128 residues), 25.7 bits, see alignment E=4.2e-09 PF07728: AAA_5" amino acids 601 to 722 (122 residues), 48.6 bits, see alignment E=4.5e-16 PF10431: ClpB_D2-small" amino acids 769 to 849 (81 residues), 98.8 bits, see alignment E=7.5e-32

Best Hits

Swiss-Prot: 73% identical to CLPB_SALTY: Chaperone protein ClpB (clpB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03695, ATP-dependent Clp protease ATP-binding subunit ClpB (inferred from 72% identity to abo:ABO_0479)

MetaCyc: 73% identical to protein disaggregase ClpB (Escherichia coli K-12 substr. MG1655)
RXN185E-10

Predicted SEED Role

"ClpB protein" in subsystem Protein chaperones or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (861 amino acids)

>ABIE51_RS04925 ATP-dependent chaperone ClpB (Lysobacter sp. OAE881)
MRMDKLTSRFQQALQDAQSLALGRDHTIIEPVHLMTALLDQNGGSTRPLLAQAGVNVPLL
RERLGEALEKLPKVTGQAGQISVGNDLSRLLNVTDKLAQQRGDAFIASELFVLAALDDNG
DVGRALKAAGASKDKLEAAIAKVRGGESVQSENAEDQRQALEKYTIDLTARAENGKLDPV
IGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIINGEVPEGLRGKRVLSL
DMGALIAGAKFRGEFEERLKAVLNDLAKNEGQIILFIDELHTMVGAGKAEGAMDAGNMLK
PALARGELHCIGATTLDEYRQYIEKDAALERRFQKVFVGEPSVEDTIAILRGLKEKYAVH
HGVEITDPAIVAAATLSNRYISDRQLPDKAIDLMDEAASRIRMEIDSKPEELDRKERRLI
QLKIQREALKKEKDAESRQRLADLEDEIARLEREFNDLEEVWKAEKATLQGATKIKEQIE
QAKLELEAAQRRQDFAKMSEIQYGTLPELEKQLRAAQEAETKGFTLLQDKVTAEEIAEVV
ARWTGIPVSKMLEGEREKLLRMEEQLHTRVVGQEEAIKVVSDAVRRSRAGLSDPNRPSGS
FLFLGPTGVGKTELCKALAEFLFDSSDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
YLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVIVMTSNL
GSQMIQEMSGDDTPQAYLQMKSAVMGVVQSHFRPEFINRLDDIVVFHPLDKQQIRQIARI
QLNGLEKRLAERGLKLVLSDDALNLLGNVGFDPVYGARPLKRAIQQQLENPLASKILAGE
FTSGDTVHVEAQGGQLVFRKD