Protein Info for ABIE51_RS04875 in Lysobacter sp. OAE881

Annotation: NAD+ synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 6 to 249 (244 residues), 101.2 bits, E=6.2e-33
• PF02540: NAD_synthase
  • amino acids 270 to 519 (250 residues), 228.7 bits, E=6.2e-72
• TIGR00552: NAD+ synthetase
  • amino acids 278 to 524 (247 residues), 186.6 bits, E=2.4e-59

Best Hits

Swiss-Prot: 74% identical to NADE_XYLFA: Glutamine-dependent NAD(+) synthetase (nadE) from Xylella fastidiosa (strain 9a5c)

KEGG orthology group: K01950, NAD+ synthase (glutamine-hydrolysing) [EC: 6.3.5.1] (inferred from 77% identity to psu:Psesu_0557)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• NAD de novo biosynthesis II (from tryptophan) (9/9 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
• aspartate superpathway (19/25 steps found)
• NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
• NAD de novo biosynthesis I (4/6 steps found)
• NAD de novo biosynthesis III (4/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (4/6 steps found)
• NAD salvage pathway I (PNC VI cycle) (4/7 steps found)
• superpathway of NAD biosynthesis in eukaryotes (9/14 steps found)
• NAD salvage pathway V (PNC V cycle) (2/5 steps found)
• NAD salvage (plants) (4/11 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5 or 6.3.5.1

Protein Sequence (544 amino acids)

>ABIE51_RS04875 NAD+ synthase (Lysobacter sp. OAE881)
MPNPLRIALAQFDFPVGAVQRNAERIAAMIEQARDEYGADIVLFPELALSGYPPEDLLLR
PSFLADCQAELERIATNVRDIVAVVGWPQAAGSVVYNAASVLRDGHVSHTYRKRELPNYA
VFDERRYFDVDPDRDDCVFDVDGVQVGLLVCEDLWFPEPLASTVAKGAELVLVPNASPFE
RDKHADRDALLAQRVQETGVGLVYVNTVGGQDAVVFDGGSVVADGDGTVHPAAAAFTDQW
LVVDFDSDSRRFSPVQWMEDGDESRDALAWRAVVRGTRDYFAKNGFKHAWLGLSGGIDSS
LVLAIAVDALGAENVTAVRMPSRYTAGMSNDLAEEQCRMQGVRLMTVPIEKPFQGFLDAL
SETFAGRPVDTTEENLQSRIRGALLMAMSNKFGGLLLTTGNKSEYAVGYATIYGDMAGGY
APLKDIYKTEVFALARWRNTVGDRVIPQAVIDRPPSAELRENQKDQDSLPPYDVLDAILL
RYVDQEQSREDIISAGFDQATVDRVLRLVRISEWKRHQAAPGPKVSRRAFGRERRYPITN
GYGH