Protein Info for ABIE51_RS03925 in Lysobacter sp. OAE881

Annotation: tRNA dihydrouridine(20/20a) synthase DusA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 336 TIGR00742: tRNA dihydrouridine synthase A" amino acids 13 to 326 (314 residues), 409.2 bits, see alignment E=6.6e-127 PF01207: Dus" amino acids 16 to 313 (298 residues), 250.6 bits, see alignment E=1.1e-78

Best Hits

Swiss-Prot: 71% identical to DUSA_XANCP: tRNA-dihydrouridine(20/20a) synthase (dusA) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K05539, tRNA-dihydrouridine synthase A [EC: 1.-.-.-] (inferred from 76% identity to psu:Psesu_2541)

Predicted SEED Role

"tRNA dihydrouridine synthase A"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (336 amino acids)

>ABIE51_RS03925 tRNA dihydrouridine(20/20a) synthase DusA (Lysobacter sp. OAE881)
MSHSPAISPDALRLSVAPMMDWTDTHCRVFHRLLAPHARLYTEMVHANAVIHGDRARLLA
MDPVEHPVALQLGGSEPELLGAAAKIGAEHGFDEINLNCGCPSDRVQAGRFGACLMREPQ
LVADSVAAMVESCDVPVTVKCRLGVDDDHDWDRFLAFIDTVANAGCGMFVIHARNAWLQG
LSPKENREVPPLRYDWAYRLKHERPQLQIVVNGGIATQDEAASHLAHTDGAMLGRAAYHD
PYLLHRLDVAWFGGELRTRGELLRALRPYVEDQLARGVYLKHITRHLLGLFAGERGGRAF
RQVLSEGAHKPGADWSLVEQAIEITESFAGERVDAA