Protein Info for ABIE51_RS01140 in Lysobacter sp. OAE881

Annotation: biotin synthase BioB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00433: biotin synthase
  • amino acids 30 to 325 (296 residues), 402.5 bits, E=5.4e-125
• PF04055: Radical_SAM
  • amino acids 63 to 218 (156 residues), 81.4 bits, E=8.9e-27
• PF06968: BATS
  • amino acids 235 to 325 (91 residues), 106.7 bits, E=5.5e-35

Best Hits

Swiss-Prot: 81% identical to BIOB_XANAC: Biotin synthase (bioB) from Xanthomonas axonopodis pv. citri (strain 306)

KEGG orthology group: K01012, biotin synthetase [EC: 2.8.1.6] (inferred from 82% identity to xal:XALc_0354)

MetaCyc: 63% identical to biotin synthase (Escherichia coli K-12 substr. MG1655)
Biotin synthase. [EC: 2.8.1.6]

Predicted SEED Role

"Biotin synthase (EC 2.8.1.6)" in subsystem Biotin biosynthesis (EC 2.8.1.6)

MetaCyc Pathways

• biotin biosynthesis I (14/15 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate II (4/4 steps found)
• biotin biosynthesis II (5/6 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate III (3/5 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.6

Protein Sequence (350 amino acids)

>ABIE51_RS01140 biotin synthase BioB (Lysobacter sp. OAE881)
MPAVSQIPLVESSPAGDLRHDWSRAEVRALFDLPFPELLHRAASVHRANFDPAEVQVSTL
LSIKTGGCPEDCGYCPQAARYHTGVEATKLMSTEAVLEKARQAKAAGASRFCMGAAWRSP
KDRDIPKVAAMISEVKSLGLETCATLGMLSADQARALKDAGLDYYNHNLDTAPEYYNAII
HTREMQDRLDTLSHVRDAGMKTCCGGIVGMGERRDQRAGLLQTLANLPAHPDSVPINRLV
QVEGTPLAGTELLDPFEFVRTVAVARILMPRSMVRLSAGRESMSDELQALCFLAGANSIF
YGEKLLTTGNPDTERDMALFDRLGLRPMPVVEQGETVHADIVEAAHHCAA