Protein Info for ABCV34_RS12290 in Castellaniella sp019104865 MT123

Annotation: DNA topoisomerase III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 898 TIGR01056: DNA topoisomerase III" amino acids 3 to 619 (617 residues), 545 bits, see alignment E=1.5e-167 PF01751: Toprim" amino acids 4 to 130 (127 residues), 32.6 bits, see alignment E=1.1e-11 PF01131: Topoisom_bac" amino acids 153 to 598 (446 residues), 366.5 bits, see alignment E=3e-113 PF13342: Toprim_Crpt" amino acids 650 to 707 (58 residues), 64.9 bits, see alignment 7.7e-22 amino acids 754 to 814 (61 residues), 68.1 bits, see alignment 7.7e-23

Best Hits

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 78% identity to bbr:BB4960)

Predicted SEED Role

"DNA topoisomerase III (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (898 amino acids)

>ABCV34_RS12290 DNA topoisomerase III (Castellaniella sp019104865 MT123)
MTKTLIIAEKPSVALDISRALGGFTREGDYFESEQFVLSSSVGHLLGLVAPNDPVRGKWS
FAHLPVIPPQFELSPADKRSTERLKLLVKLLKRKDVGAIINACDAGREGELIFRYIVQYS
GVKKPIQRLWLRSMTQAAIREAFANLRDDETMKPLEAAARSRAEADWLVGINGTRAMTAF
NSKDGGFFKTPVGRVQTPTLAIVMEREDRIRQFITRDYWEVHATFVAAAGLYEGRWFDPA
FKKNEQDPDARDSRLWSASAAQTIVAACREQPGTVTEESKPSTQMSPALFDLTTLQREAN
SRFGFSAKTTLSLAQTLYERHKALTYPRTDSRYLPQDYVSTVQQTMQGLSDSESGVARSV
RPFAAQVVAQDWVKPNRRIFDDKKVSDHFAIIPTLQVPRELSDAEFKIYELVTRRFLSVF
FPAAEFRITTRITQVSGHHFKTDGKVLVSPGWLAIYGREAQGDDASLIAVAEGERVRTED
IEARALVTKPPARFNEATLLSAMEGAGKLVDDEALREAMSERGLGTPATRAAIIEGLLNE
GYLRREGRDLIPSAKARQLMTLLAGLGVNELTSPELTGEWEHQLKLIEQRQLDRETFMRE
IAQMTQVIVKRAKEYERDTVPGDYATLKTPCPKCGGVVKENYRRYACTVCDFSIGKHPGG
RTFEVPEVEELLAKRTLGPLTGFISKMGRPFAAVLRITDEFKLEFDFGQKEDEDAEAVDF
SGQTPLGACPKCGGRVFEHGMNYVCEHSVGPAKTCDFRSGKVILQQEIPAEQMSQLLATG
KTKLFDGFVSSRTNRKFKAYLVRQDDGKIGFEFEARAPKPGAKAAAGKPAAKTAATAKAG
AAKASSTAKASAAKTTKSAGKTAKSTRAAASSKSAAKTGATTARKAAAESQDDDSPAF