Protein Info for DvMF_2510 in Desulfovibrio vulgaris Miyazaki F

Annotation: tRNA pseudouridine synthase B (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 TIGR00431: tRNA pseudouridine(55) synthase" amino acids 9 to 215 (207 residues), 198.1 bits, see alignment E=7.3e-63 PF01509: TruB_N" amino acids 31 to 178 (148 residues), 161.8 bits, see alignment E=7.8e-52

Best Hits

Swiss-Prot: 100% identical to TRUB_DESVM: tRNA pseudouridine synthase B (truB) from Desulfovibrio vulgaris (strain Miyazaki F / DSM 19637)

KEGG orthology group: K03177, tRNA pseudouridine synthase B [EC: 5.4.99.12] (inferred from 100% identity to dvm:DvMF_2510)

Predicted SEED Role

"tRNA pseudouridine synthase B (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DN09 at UniProt or InterPro

Protein Sequence (308 amino acids)

>DvMF_2510 tRNA pseudouridine synthase B (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MAVLPAQQHGLLVLNKPQGPTSAQCIARVKRLGQKKIGHAGTLDPMARGVLLVLLGHATK
ISGHLMADGEKVYLGTLRLGTTTDTWDAEGSVTATAPYDHVTPDDIRREVENWLGSTEQE
VPPYSAAKHQGQPLYKLSRAGRETPVKTKTVEISRAQVVSCDLPSATFRVTCSSGTYIRS
LAHSLGMRLGCGAMLTELTREYSHPFGIDEAHELDAVLAEPERLPERVIPVTRALPHWPK
LRISAAQEAGVRNGMIVPYLPEAMAELPFAEGLKAIMLAPDDTPVALAETRIVNAQPVWT
VLRGLWSQ