Protein Info for DvMF_1990 in Desulfovibrio vulgaris Miyazaki F

Annotation: Pyruvate, water dikinase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01326: PPDK_N
  • amino acids 171 to 247 (77 residues), 31.8 bits, E=9.5e-12
  • amino acids 304 to 468 (165 residues), 63.7 bits, E=1.9e-21
• PF00391: PEP-utilizers
  • amino acids 523 to 596 (74 residues), 37.5 bits, E=1.5e-13

Best Hits

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 100% identity to dvm:DvMF_1990)

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• gluconeogenesis I (10/13 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (12/17 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (18/26 steps found)
• superpathway of N-acetylneuraminate degradation (14/22 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 2.7.9.2

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See B8DQ69 at UniProt or InterPro

Protein Sequence (931 amino acids)

>DvMF_1990 Pyruvate, water dikinase (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MGLFCWLPFRRKRRDEERAAAEEAFAARYHRFKLVLNAWAKLQEVMTEMELALCCVHPFG
MQTVRSLCTRITTQVYQCIRQLDELAPGRYPELFARFDAIQAEVSAIVYGRPATMDGPLA
LPLGHPDLTLPDLADPALTRLGELRAALAPRDGEAAAPATPVTSGMSGGGVPDGFIITAA
ACQRLFRHQALQEEIGRRVQASGGIRPDNLLDLSPSIVGLIQAAEVPDEVADALMDALYA
LRKRMAARPGVQERDMRLLLRGRVWPQGSTGAAENTDDDAGGMTLWGPSIPLFEQGSGPD
AQSDRQRVLDALRATLALKYSPQAIAYRRNRGLRDAGTCVCVGCFAVEAPVAGGIAYTGN
PLHARDTSVHVYAARGLSEAVEDGAHDVDAYHVCRVGGSVRLRSIAPRAEAATGEVLPVL
PVLDDAAARAVAELALAVEERCGLPQEIDWLLEPDGSVSLLHARPLPAPRAHLPESYAES
EAEDAAQTECPPPALLDGGITAGPGVVAGPVHIVRQRDDVRSFPDGAVLVVERDLADWGM
LLDRASAVVAERGRISSQLAAVAREFGKPAVFGPFRQGSPLDLLEQGAMVTVHGDVGVVY
PGRIEAFIESAPHECNFMPGSPVLQALDAAAHHMLPLTLDPDSPDFKSANCRTFHDLGRF
CHEKAVAEMFRFGSQQRHAPQRVKQLQCDVPKQFWVVNLDDGFDGDVNGPLVPIARVASV
PMRTLWRGMNAVPWQGPPPVDAKGFLSVMFEATANPHLDPAAQSAFFTERNYFMVSRNYC
SLHSRFGFHFVAVESLVGDRTPENYVVFQLRGGAANIERRVRRVEFVAGLLGEFGFACTV
RRDALSARIENLPQPDALRMLLVAGYMTIHTRQLDMIMNDGPQLAERRASMLADCRRLLE
EADLAAAAEAEQAMSGDDARPRRAVDPAAPI