Protein Info for DvMF_1987 in Desulfovibrio vulgaris Miyazaki F

Annotation: Pyruvate, water dikinase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01326: PPDK_N
  • amino acids 144 to 464 (321 residues), 277.9 bits, E=1.2e-86
• PF00391: PEP-utilizers
  • amino acids 538 to 608 (71 residues), 57.3 bits, 9.5e-20

Best Hits

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 100% identity to dvm:DvMF_1987)

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• gluconeogenesis I (10/13 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (12/17 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (18/26 steps found)
• superpathway of N-acetylneuraminate degradation (14/22 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 2.7.9.2

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See B8DQ66 at UniProt or InterPro

Protein Sequence (918 amino acids)

>DvMF_1987 Pyruvate, water dikinase (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MGGFFHTLGALFRRQDAPAQDGGVPDAETERLRALYRERCSRFRLLLSANKSALEIMADM
EEALRGVRPFGMTYVRGACTRACASVFQIVRHLNALSDDRWPELETRFNEIRAAIEAVVA
PHVRAGGGPLVLPIAETGVDMADQTGGKMANLGEIRRRLGITVPDGFVVTATGYQRFMEA
GGLQEEIDRRVQAADATRLDEVFALSASIQQLILAAPVPDDLAGAITAQVERLAGPPQDA
RTGPHPGINACSGAEVRFAVRSSAVGEDSLDASFAGQYRSELNVPPEDVLDTFKDIVASK
YGVTAMTYRLARGIPDEDVPMCVGCLVMVDAVAGGVAYSRNPLDLRDNTVVINAVPGLPK
AVVDGSFSPDVFVVSREVPMQVAQRQIAAKPQRFVRDPIEGVRLEDMPSDMVDAPCIDDA
RALEIASLAAGFEVFYGEPQDIEWAVCPEGVLTILQSRPLREARHDELPEGECAPVAGAD
GADATAADAAAETGPLPGGYNCGESGPPVLLLGGTPVSPGAGAGLAHVVRKDMDLLSFPD
GGVLVIERALPRWAPLLSRASAVVAEAGGAAGHLASVAREYGVPALFGLPRAAELLEHAG
IVTVDADNRRVHPGRIEDLLGHAPRRNLMEGSPVHTALAEAARLVLPLNLLDPESPRFAP
AHCRTLHDITRFCHEKSVEVLFGVADHMGATGPGTQRLGKQLRVNGTRLQYWLLDIDGGF
RKPVPGPVVDLADIASAPMLALWDGMTAVPWAGPPATDARGFLSVMMESTVNPDLEPTAA
TTLANRNFLMISRDYCNLQARFGYHFCTVEAMATDNPHENYVTFQFKGGAASADRRALRA
RFVGEVLEARGFRADVKDDALFASLEGLPKDRILTLVRGVGYLLIHTRQIDMVAHNERVF
GPIRDRIAADLARIAPVT