Protein Info for DvMF_1590 in Desulfovibrio vulgaris Miyazaki F

Annotation: glutamine synthetase catalytic region (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 726 PF12437: GSIII_N" amino acids 33 to 194 (162 residues), 255.7 bits, see alignment E=2.1e-80 PF00120: Gln-synt_C" amino acids 203 to 401 (199 residues), 72.8 bits, see alignment E=4.2e-24 PF18318: Gln-synt_C-ter" amino acids 524 to 641 (118 residues), 152.8 bits, see alignment E=5.1e-49

Best Hits

KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 100% identity to dvm:DvMF_1590)

MetaCyc: 56% identical to glutamine synthetase subunit (Ruminococcus albus 8)
Glutamate--ammonia ligase. [EC: 6.3.1.2]

Predicted SEED Role

"Glutamine synthetase type III, GlnN (EC 6.3.1.2)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Glutamine synthetases or Peptidoglycan Biosynthesis (EC 6.3.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.2

Use Curated BLAST to search for 6.3.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DRZ9 at UniProt or InterPro

Protein Sequence (726 amino acids)

>DvMF_1590 glutamine synthetase catalytic region (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MSGIQARLNAISAITNYKPSAAPMNFAETKPTELFGCNVFNDKVMKDRLPKTVYKSLKKT
IELGEKLDPSIADVVANAMKDWAIEKGATHFTHVFYPLTGLTAEKHDAFLVPDGKGGALS
EFSGKLLIQGEPDASSFPSGGLRATFEARGYTAWDVTSPAYLLENPNGTFLCIPTAFVSW
TGEALDKKTPLLRANQALNKQAQRVLKLFGTETKLPLVSYAGPEQEYFLIDRNFAFARPD
LHICGRSLFGAKPAKGQEFEDQYFGVIPRRVLSFMMEVERELFKLGVPVKTRHNEVAPSQ
YEIAPIFETSNLATDHNQLIMTVLRSVAKRYGMVCLLHEKPFAGINGSGKHLNYSIGNAD
LGSLFDPGESPHENAQFLVFCAAAIRAVHKYGALLRATVASASNDHRLGANEAPPAIMSV
YLGEQLTDVFEQIKTGKVNGCKKACVMNIGVDVLPPLPMDPGDRNRTSPFAFTGNRFEFR
AVGSSMSIAGPQVALNTMMAESLDYIATELEKATKGDPAKLNEAVQKLLQKIMKEHDKVI
FNGDGYSEEWHKEAERRGLPNLKTTPDALPVLSSPEVVKLFTSYGVFSEAEVKSREEIYL
EQYCKTVKTEANLVIRMARTIIFPAAMRYQGELAATCANLKAAGHDYKVVTLEDVTTKLR
AMQAAVGELEKKLDHEAASTHAEAKHMCDVILPAMLKVREYADALEAVVADDLWALPSYQ
EMLFIK