Protein Info for DvMF_1485 in Desulfovibrio vulgaris Miyazaki F

Annotation: glutaminyl-tRNA synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 569 TIGR00440: glutamine--tRNA ligase" amino acids 37 to 562 (526 residues), 796.3 bits, see alignment E=7.8e-244 PF00749: tRNA-synt_1c" amino acids 37 to 345 (309 residues), 374.8 bits, see alignment E=4.2e-116 PF03950: tRNA-synt_1c_C" amino acids 348 to 448 (101 residues), 113.6 bits, see alignment E=6.2e-37 PF20974: tRNA-synt_1c_C2" amino acids 465 to 540 (76 residues), 86.8 bits, see alignment E=2e-28

Best Hits

Swiss-Prot: 63% identical to SYQ_RHOBA: Glutamine--tRNA ligase (glnS) from Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)

KEGG orthology group: K01886, glutaminyl-tRNA synthetase [EC: 6.1.1.18] (inferred from 100% identity to dvm:DvMF_1485)

MetaCyc: 62% identical to glutamine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glutamine--tRNA ligase. [EC: 6.1.1.18]

Predicted SEED Role

"Glutaminyl-tRNA synthetase (EC 6.1.1.18)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DLQ7 at UniProt or InterPro

Protein Sequence (569 amino acids)

>DvMF_1485 glutaminyl-tRNA synthetase (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MSDHNASEPDRKAEPGLDFIRALITADNANGRFDSRVHTRFPPEPNGYLHIGHAKSICLN
FGVAREFGGKCNLRFDDTNPLKESQEYVDSIREDVKWLGGDWQGREFYASDYFEQLYQYA
EQLILAGKAYVDSLSADEIREHRGTLTAPGKESPYRNRSVEENLDLFRRMRAGEFKDGEH
VLRAKIDMASPNVVMRDPTLYRIRHAHHHRTGDAWCIYPMYDYTHCISDSIEGITHSICT
LEFVNNRELYDWTLDTLGVYHPQQLEFARLNLTYTVLSKRKLIQLVEGGYVNGWDDPRMP
TISGLRRRGVTPEALQDFCARIGVAKADSVVDFSLLEFCMREHLNAVAPRVMGVLDPIKV
VIENYPDDQVETFDMPYHPEDTSHGSRTVPFSKVLYIERDDFREDPPKKYHRLAPGAEVR
LRYAYYITCREVVKDADGNITELRCTYDPATKGGWSQDGRKVKGTIHWVSAAHALHAEVR
LYEHLFSVENPNAAEEGKTFVDYLNPDSLKTVTAMLEPALADVAPGTRVQFERIGYFCAD
KDGTPGKPVFNRTVGLRDSWAKIEKKEGA