Protein Info for DvMF_0787 in Desulfovibrio vulgaris Miyazaki F

Annotation: (p)ppGpp synthetase I, SpoT/RelA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 722 TIGR00691: RelA/SpoT family protein" amino acids 26 to 715 (690 residues), 804.5 bits, see alignment E=4e-246 PF13328: HD_4" amino acids 26 to 175 (150 residues), 176.2 bits, see alignment E=1.5e-55 PF01966: HD" amino acids 45 to 144 (100 residues), 45.4 bits, see alignment E=3.3e-15 PF04607: RelA_SpoT" amino acids 235 to 344 (110 residues), 145 bits, see alignment E=3.8e-46 PF02824: TGS" amino acids 387 to 446 (60 residues), 83.3 bits, see alignment 3.5e-27 PF19296: RelA_AH_RIS" amino acids 459 to 623 (165 residues), 62.5 bits, see alignment E=2.1e-20 PF13291: ACT_4" amino acids 640 to 716 (77 residues), 71.6 bits, see alignment E=2.4e-23 PF01842: ACT" amino acids 649 to 709 (61 residues), 39.8 bits, see alignment 1.1e-13

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 100% identity to dvm:DvMF_0787)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DL41 at UniProt or InterPro

Protein Sequence (722 amino acids)

>DvMF_0787 (p)ppGpp synthetase I, SpoT/RelA (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MIRIQDVLDKVASVNKNADLALIQKAYVYAAAAHAGQTRLSGEPYLSHPLAVAYSLAEMR
FDEAAIAAGLLHDTVEDTTVSIEDIDTLFGEEVADVVDGVTKISQMTFESKEEAQAENIR
KMILAMAHDIRVLIVKLADRLHNMRTLGFQKSHKQKLIAQETMDIYAPLANRLGLHRIKL
ELEDLSFRYLKPDVYAQIDEWLDTNQVVERHYIERVITRIREILEKNEIKGEVRGRIKHK
YSIYKKMVQQDLTLDDMHDIIAFRVIVGDLRDCYAVLGLMHAQWKPVHGRFKDYISMPKA
NGYRSLHTTVIGPEGERMEIQIRTEEMHRLAEHGVASHWLYKEGSRVNPRDLEQFTWLHE
ILDRQKMETDSKEFMQSLRLDLFKDEVYVFTPRGDVKELPEGATPIDFAYLIHTEVGNHC
AGAKVNGKLVPLGTALKSGDTIEVITDNSRRPSRDWLKFVKTAKARSRIQHYIRTEERER
SIALGREMLEKEGRRMGINITKALRDGDLLPVIKELSLGSVEDLLSNVGYARYTPKKVLK
RLLPKDEEAAAEEAAKAAEASAASAATREANRRADSVSIKGVDDILVRFARCCNPLPGDP
IIGFISRGRGVTVHTSDCANVQTMEPERLISVFWDGQEDKPYPARIHLVCRNEKGVLAQI
TALLAEENVNIDSGNMRSMVDGKSEVDFTVEVRDVAHLYRALDKLRKLPTVVEVLRSTST
ME