Protein Info for DvMF_0320 in Desulfovibrio vulgaris Miyazaki F

Annotation: Formate dehydrogenase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 PF04879: Molybdop_Fe4S4" amino acids 7 to 52 (46 residues), 46.9 bits, see alignment 3.3e-16 PF00384: Molybdopterin" amino acids 62 to 482 (421 residues), 123.6 bits, see alignment E=1.5e-39 PF01568: Molydop_binding" amino acids 576 to 655 (80 residues), 42.2 bits, see alignment E=1.1e-14

Best Hits

KEGG orthology group: K08352, thiosulfate reductase [EC: 1.-.-.-] (inferred from 100% identity to dvm:DvMF_0320)

MetaCyc: 74% identical to thiosulfate reductase (cytochrome) (Desulfovibrio vulgaris)
RXN-8339 [EC: 1.8.2.5]

Predicted SEED Role

"Thiosulfate reductase precursor (EC 1.-.-.-)" in subsystem Anaerobic respiratory reductases (EC 1.-.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.- or 1.8.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8DPJ0 at UniProt or InterPro

Protein Sequence (700 amino acids)

>DvMF_0320 Formate dehydrogenase (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MAGRQQVYSVCGMCSVRCPIVVEVEDGKAVRIEGNPKSPLKGSLCARGAAGIALEMDTER
PQRPLIRVGERGEGKWREASWDEALDYVAGKLKDITAAHGGRSVLFSDRGGPFVDLHQAF
MRGLGSPNYCNHDSACARNVQHAAKSVMGIGRKEVVYDYRNCKHLVLQTRNILEAINVAE
ANAVLDGMEKGCKLSVVDIRATYTAGKADNFYMVRPGSDLAFNLAVIHVLINEGLYNKPY
VEAHVQDLDALAQHVAACTPQWAEGETGVPAAAITALARQLAEAAPNVIWHPGWMVARYD
DSFHVCRTAYIINALLGSIGAKGGLPIANKPGDVGRKGLKKLVDLYPAPEEKRADGVGWR
HTQFDGGPGLINLAYDAIATGDPYPVKAYICYRHDPLMAMPDAEAMKRKWEGLDLLVSVT
FSWSDTAWHSDVVLPLSTYLSRESIIASKGGLKPQFFVRKRAMQPTLDTRADWEILCGLA
KRLGMDKLAFDRIEDIWEYQLQPTGVTMADFDAKGFVELCDAPKYREDAELKFATSSGKL
EMISPKWTKAGEPSLRPYAAPPAPPQGMFRIAFGRTALHTQGHTVNNPLLAEQMPTNVAW
INTARAAELGIADGELLEVLDARGTAAGRIRAFVTDFVHPEALFMVHGFGHRLPVESRAR
GKGVADQDLMPGGLERYDRAGGGISMQEHFVAVRKAAARA