Protein Info for DvMF_0276 in Desulfovibrio vulgaris Miyazaki F

Annotation: aspartate kinase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00656: aspartate kinase, monofunctional class
  • amino acids 1 to 401 (401 residues), 438.9 bits, E=1.9e-135
• PF00696: AA_kinase
  • amino acids 3 to 230 (228 residues), 174.6 bits, E=4.3e-55
• TIGR00657: aspartate kinase
  • amino acids 63 to 401 (339 residues), 389.3 bits, E=2.9e-120
• PF01842: ACT
  • amino acids 265 to 325 (61 residues), 39.8 bits, E=4.7e-14
• PF13840: ACT_7
  • amino acids 338 to 398 (61 residues), 58.2 bits, E=8.9e-20

Best Hits

Swiss-Prot: 55% identical to AKLYS_PSEU5: Aspartate kinase Ask_LysC (lysC) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K00928, aspartate kinase [EC: 2.7.2.4] (inferred from 100% identity to dvm:DvMF_0276)

MetaCyc: 54% identical to aspartokinase (Halomonas elongata DSM 2581)
Aspartate kinase. [EC: 2.7.2.4]

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis (EC 2.7.2.4)

MetaCyc Pathways

• superpathway of L-isoleucine biosynthesis I (12/13 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• aspartate superpathway (20/25 steps found)
• L-lysine biosynthesis VI (7/7 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-homoserine biosynthesis (3/3 steps found)
• L-lysine biosynthesis I (7/9 steps found)
• chorismate biosynthesis II (archaea) (9/12 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (9/12 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• spermidine biosynthesis II (3/4 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (13/18 steps found)
• L-lysine biosynthesis II (6/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (6/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (6/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (5/8 steps found)
• L-methionine biosynthesis IV (2/4 steps found)
• 3-dehydroquinate biosynthesis II (archaea) (4/7 steps found)
• ectoine biosynthesis (2/5 steps found)
• norspermidine biosynthesis (2/6 steps found)
• superpathway of polyamine biosynthesis III (3/8 steps found)
• cremeomycin biosynthesis (2/7 steps found)
• grixazone biosynthesis (2/8 steps found)
• platensimycin biosynthesis (3/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Lysine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.4

Sequence Analysis Tools

See B8DPE6 at UniProt or InterPro

Protein Sequence (409 amino acids)

>DvMF_0276 aspartate kinase (RefSeq) (Desulfovibrio vulgaris Miyazaki F)
MRILVQKFGGTSVANLECMKQVREKVRRALRDGYKPVVVLSARSGETNRLLALAEEWSTD
PDPAEVDALIATGEQVSVALFSMLLKDSGIKARSMTGFQVPITTSDAFGRARIMDIDASR
LRKELETHDVIVVAGFQGVTSEGRVTTLGRGGSDTSAVALAAALGSVECEIYTDVDGVYT
TDPNLCSTARKLDRVSYDEMLEMASMGAKVLQIRSVEFAKKYKVPVHVRSTFTDTPGTMV
TQEDCEMEAVLVSGIAYDKDQARVTLRSVPDKPGVSAAIFGPLSQQGILVDMIVQNPSRD
GVTDMTFTVPRKDLKKTLALMEEIRARTGAQEVLHDTQVAKVSAIGVGMRNHSGVAAKAF
AALHAENINILMISTSEIKITCLIEEKYTELAVRTLHDAFGLNHDLGNA