Protein Info for Shewana3_4102 in Shewanella sp. ANA-3

Annotation: coproporphyrinogen oxidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 48% identical to KDTA_ECOLI: 3-deoxy-D-manno-octulosonic acid transferase (waaA) from Escherichia coli (strain K12)

KEGG orthology group: K02527, 3-deoxy-D-manno-octulosonic-acid transferase [EC: 2.-.-.-] (inferred from 100% identity to shn:Shewana3_4102)

MetaCyc: 48% identical to KDO transferase (Escherichia coli K-12 substr. MG1655)
KDOTRANS2-RXN [EC: 2.4.99.13]; KDOTRANS-RXN [EC: 2.4.99.13, 2.4.99.12]

Predicted SEED Role

"Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (EC 2.4.99.12) [often with (EC 2.4.99.13) also]" (EC 2.4.99.12, EC 2.4.99.13)

MetaCyc Pathways

superpathway of (Kdo)₂-lipid A biosynthesis (15/17 steps found)
Kdo transfer to lipid IV_A (Brucella) (2/2 steps found)
Kdo transfer to lipid IV_A (E. coli) (2/2 steps found)
Kdo transfer to lipid IV_A (H. pylori) (2/2 steps found)
Kdo transfer to lipid IV_A (P. gingivalis) (2/2 steps found)
Kdo transfer to lipid IV_A (P. putida) (2/2 steps found)
Kdo transfer to lipid IV_A (generic) (2/2 steps found)
Kdo transfer to lipid IV_A (Haemophilus) (1/2 steps found)
Kdo transfer to lipid IV_A (Vibrio cholerae serogroup O1 El Tor) (1/2 steps found)
Kdo transfer to lipid IV_A (Chlamydia) (2/5 steps found)
superpathway of Kdo₂-lipid A biosynthesis (15/25 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.- or 2.4.99.12 or 2.4.99.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L2Q0 at UniProt or InterPro

Protein Sequence (441 amino acids)

>Shewana3_4102 coproporphyrinogen oxidase (RefSeq) (Shewanella sp. ANA-3)
MLTLPLFIRQKLISTIFMNRFFYSVLLYLLSPLLIVYLAFRAIKSPDYRGRWDERFGLTR
LKSTDLLIHSVSMGETLAAIPLIRQIQQAHPQLKITVTTSSPTGSAEVRKAFGDQVQHCY
LPFDLPWCVNRFLRQLSPKWCIIMETELWPNLVALAAKRGVRLMLANARLSAKSAAQYAK
RPHLSRPMLQRLDVVAVQTQAEAQRFIDLGVPADRVTVCGSLKFDLSITPERLTLARELR
LTWGKEAAPVWVAGSVHPGEFDAMLSAHKQLLAKWPEALLVIAPRHPEQFAAVADVVASQ
GFEFVRRSDAQAITATTQVLVGDTMGELLTFYGAADQAFVGGTLIENGGHNPLEPVAMGV
PVMVGPNHWDFAQITQMLADAGGLRIVSSGQELGENLIQYFEQPALRQQAAEAGLAVVEA
NRGALQRQFALAESLLSRQTT