Protein Info for Shewana3_4056 in Shewanella sp. ANA-3

Annotation: gamma-glutamyl kinase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00696: AA_kinase
  • amino acids 7 to 233 (227 residues), 141.7 bits, E=1.7e-45
• TIGR01027: glutamate 5-kinase
  • amino acids 7 to 352 (346 residues), 328.2 bits, E=3.4e-102

Best Hits

Swiss-Prot: 63% identical to PROB1_PSEHT: Glutamate 5-kinase 1 (proB1) from Pseudoalteromonas haloplanktis (strain TAC 125)

KEGG orthology group: K00931, glutamate 5-kinase [EC: 2.7.2.11] (inferred from 100% identity to she:Shewmr4_3848)

MetaCyc: 35% identical to glutamate 5-kinase (Escherichia coli K-12 substr. MG1655)
Glutamate 5-kinase. [EC: 2.7.2.11]

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-ornithine biosynthesis II (3/3 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-N^δ-acetylornithine biosynthesis (5/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.7.2.11

Use Curated BLAST to search for 2.7.2.11

Sequence Analysis Tools

See A0L2K4 at UniProt or InterPro

Protein Sequence (368 amino acids)

>Shewana3_4056 gamma-glutamyl kinase (RefSeq) (Shewanella sp. ANA-3)
MTDSPRKRIVVKVGSALIAPHKQGCSSHYLLGIAQFITYCRVQGIQVVLVSSGSVAAGWH
HFEGQAQPSVTVKKAMAAAGQADMMATWNKLFDFPTAQLLLTHGDLRNRERYISIRDTIF
SLLEHGLMPIINENDAVTADKLKVGDNDNLSAMVAAAADADTLVICSDVDGLYDQNPHEH
PNAKLIKQVTEINADIYAMAGGVSSDVGTGGMRTKIQAAEKAISHGIETFIINGFNADSF
SQLLKGQNPGTLFTPYEKPMQEHLHWMTHTSQAQGEVIVEDDFDLALDQHSEQLTSDDVV
EVKGDFSVGDTILVRKGDGTKLAKAKSNYSSCLLSFITEQDDQAFASEFQQKTGPIISDK
NIAILKSI