Protein Info for Shewana3_3680 in Shewanella sp. ANA-3

Updated annotation (from data): Uridine phosphorylase (EC 2.4.2.3)
Rationale: Specifically important for utilizing Cytidine and Uridine. Automated validation from mutant phenotype: the predicted function (URPHOS-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: uridine phosphorylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01718: uridine phosphorylase
  • amino acids 4 to 251 (248 residues), 362.8 bits, E=3.8e-113
• PF01048: PNP_UDP_1
  • amino acids 18 to 234 (217 residues), 133.1 bits, E=5.5e-43

Best Hits

Swiss-Prot: 76% identical to UDP_ECOLI: Uridine phosphorylase (udp) from Escherichia coli (strain K12)

KEGG orthology group: K00757, uridine phosphorylase [EC: 2.4.2.3] (inferred from 98% identity to sbm:Shew185_0467)

MetaCyc: 76% identical to uridine phosphorylase (Escherichia coli K-12 substr. MG1655)
Pyrimidine-nucleoside phosphorylase. [EC: 2.4.2.2, 2.4.2.3]

Predicted SEED Role

"Uridine phosphorylase (EC 2.4.2.3)" (EC 2.4.2.3)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleosides degradation (6/6 steps found)
• pyrimidine deoxyribonucleosides degradation (3/3 steps found)
• pyrimidine ribonucleosides degradation (2/2 steps found)
• superpathway of pyrimidine ribonucleosides degradation (2/5 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.2

Use Curated BLAST to search for 2.4.2.2 or 2.4.2.3

Sequence Analysis Tools

See A0L1I0 at UniProt or InterPro

Protein Sequence (252 amino acids)

>Shewana3_3680 Uridine phosphorylase (EC 2.4.2.3) (Shewanella sp. ANA-3)
MADVFHLGLTKAMLDGATLAIVPGDPERVKRIAELMDNATFLASHREYTSYLAYADGKPV
VICSTGIGGPSTSIAVEELAQLGVNTFLRVGTTGAIQPHVNVGDVIVTQASVRLDGASLH
FAPMEFPAVANFECTTAMVAACRDAGVEPHIGVTASSDTFYPGQERYDTVTGRVTRRFAG
SMKEWQDMGVLNYEMESATLFTMCATQGWRAACVAGVIVNRTQQEIPNEATMKQTEVSAV
SIVVAAAKKLLA