Protein Info for Shewana3_3405 in Shewanella sp. ANA-3

Annotation: glutamyl-tRNA synthetase, class Ic (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 299 transmembrane" amino acids 24 to 42 (19 residues), see Phobius details TIGR03838: glutamyl-queuosine tRNA(Asp) synthetase" amino acids 15 to 272 (258 residues), 361.3 bits, see alignment E=1.6e-112 PF00749: tRNA-synt_1c" amino acids 18 to 250 (233 residues), 142.5 bits, see alignment E=7.3e-46

Best Hits

Swiss-Prot: 88% identical to GLUQ_SHEON: Glutamyl-Q tRNA(Asp) synthetase (gluQ) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01894, glutamyl-Q tRNA(Asp) synthetase [EC: 6.1.1.-] (inferred from 100% identity to shn:Shewana3_3405)

MetaCyc: 58% identical to glutamyl-Q tRNAAsp synthetase (Escherichia coli K-12 substr. MG1655)
2.4.1.M62 [EC: 2.4.1.M62]

Predicted SEED Role

"glutamyl-Q-tRNA synthetase" in subsystem Queuosine-Archaeosine Biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.-

Use Curated BLAST to search for 2.4.1.M62 or 6.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L0Q9 at UniProt or InterPro

Protein Sequence (299 amino acids)

>Shewana3_3405 glutamyl-tRNA synthetase, class Ic (RefSeq) (Shewanella sp. ANA-3)
MMATHSSNISALHPYVGRFAPSPSGALHFGSLIAALGSYLRARSLGGKWLIRVEDIDPPR
EVAGAADDILRTLEAYGFEWDDTVLYQSARTEAYQAKLDELLAEDNAYFCQCSRKQIQAM
GGIYDGRCHQLATPHQSGAIRIVNRQQVAEFSDNLMGKVVVDHAFAAEDFIIKRSDGLYA
YQLAVVLDDAYQGITEVVRGYDLIEASCRQLSLYQTFGLSAPQWLHLPLACLTPGFKLSK
QNHAQAIDKQHPQASLNAALAFLGQAPVEPTAAPQMLAQAVAQFQLSAIPKQREILIAP