Protein Info for Shewana3_3324 in Shewanella sp. ANA-3

Annotation: peptidase M17, leucyl aminopeptidase domain-containing protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 511 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF02789: Peptidase_M17_N" amino acids 42 to 163 (122 residues), 75.1 bits, see alignment E=4.8e-25 PF00883: Peptidase_M17" amino acids 196 to 500 (305 residues), 390.8 bits, see alignment E=4.8e-121

Best Hits

Swiss-Prot: 96% identical to AMPA1_SHEON: Probable cytosol aminopeptidase 1 (pepA1) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 100% identity to she:Shewmr4_0802)

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.1

Use Curated BLAST to search for 3.4.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L0H9 at UniProt or InterPro

Protein Sequence (511 amino acids)

>Shewana3_3324 peptidase M17, leucyl aminopeptidase domain-containing protein (RefSeq) (Shewanella sp. ANA-3)
MKNKNKFLLSVMALACLNSLNANAETFSFDTRNSLNSDTLVLFQSADSTTYSLDFLPQST
QDQLNLAVADNSFAGKRGEILEILVPSEIDAKRVLLVGIGDAKTLTPGEINTLGGNIAAK
LETVPQATVRVLTQGLSNAPLFGSELAHGIELRSYRYTQFKASNRAEKNYQIGVDDLSQN
QKHHKNLQAIEAGVFLARDLTNAPAGIMYPESFANEARKLKSLGVKVTVLEAKDIERLNL
GALAAVGKGSERPPKLVVAHWPGSKEAPIALVGKGITFDSGGYNIKATGTSIARMKSDMA
GAATVLGTVKAMAIQKAPVNLVAIMPMAENMVSGHAMIPGDVIKTAQGLTVEVLNTDAEG
RLVLADGLWYARENYRPSIIVDVATLTGSKVGALGSVYAGLFTDFEPLVQQLTFAGQQVG
EKVWRLPLDQAYDDELKSTIADLKNTGKEGSAGASSAAMFLKRFAGEQPWAHLDIAGNAL
TATDTAVVPAGATGYGVRLLSTWLTQPKAQN