Protein Info for Shewana3_3156 in Shewanella sp. ANA-3
Annotation: phosphoribosylaminoimidazole carboxylase ATPase subunit (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 53% identical to PURK_ECOLI: N5-carboxyaminoimidazole ribonucleotide synthase (purK) from Escherichia coli (strain K12)
KEGG orthology group: K01589, 5-(carboxyamino)imidazole ribonucleotide synthase [EC: 6.3.4.18] (inferred from 100% identity to shn:Shewana3_3156)MetaCyc: 53% identical to 5-(carboxyamino)imidazole ribonucleotide synthase (Escherichia coli K-12 substr. MG1655)
5-(carboxyamino)imidazole ribonucleotide synthase. [EC: 6.3.4.18]
Predicted SEED Role
"Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21)" in subsystem De Novo Purine Biosynthesis (EC 4.1.1.21)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
- inosine-5'-phosphate biosynthesis I (6/6 steps found)
- inosine-5'-phosphate biosynthesis II (5/5 steps found)
- inosine-5'-phosphate biosynthesis III (5/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.1.1.21
Use Curated BLAST to search for 4.1.1.21 or 6.3.4.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0L011 at UniProt or InterPro
Protein Sequence (365 amino acids)
>Shewana3_3156 phosphoribosylaminoimidazole carboxylase ATPase subunit (RefSeq) (Shewanella sp. ANA-3) MTQASAKPKVWVLGNGQLGAMLTHAGEPLAIDVRAVDIMTPTDEILPLAPHDIITAEREQ WPESALSLQLSTHQHFVNGPVFSRLADRYSQKSLLDQIQVPTAPWTLVDDKTDVENLYQA FGPRVLMKRRTGGYDGKGQHWLKQAEAGEIPQDWRNLAIAEQAINFDEEVSLVGVRTREG QCVFYPLTLNLHQDGILMASISPLTRLNHLQAQAEGMLSAIMHELEYVGVMAMECFRVGD KLLVNELAPRVHNSGHWTQAGTHMDQFQLHLRALCGIAIPQPQVNFQCVMVNLIGIENDP RWLNLPNAELYWYNKEVRPGRKVGHLNLSVPNTTVLKDSISALQTWMPSQYQAPLAWILA EFAKA