Protein Info for Shewana3_3112 in Shewanella sp. ANA-3

Updated annotation (from data): N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
Rationale: Specifically important for utilizing N-Acetyl-D-Glucosamine. Automated validation from mutant phenotype: the predicted function (NAG6PDEACET-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: N-acetylglucosamine 6-phosphate deacetylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00221: N-acetylglucosamine-6-phosphate deacetylase
  • amino acids 3 to 372 (370 residues), 306.4 bits, E=1.4e-95
• PF01979: Amidohydro_1
  • amino acids 46 to 366 (321 residues), 82.8 bits, E=2.9e-27

Best Hits

Swiss-Prot: 55% identical to AGAA2_SHESA: N-acetylgalactosamine-6-phosphate deacetylase (agaAII) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K01443, N-acetylglucosamine-6-phosphate deacetylase [EC: 3.5.1.25] (inferred from 100% identity to shn:Shewana3_3112)

MetaCyc: 55% identical to N-acetylgalactosamine-6-phosphate deacetylase (Shewanella sp. ANA-3)
N-acetylglucosamine-6-phosphate deacetylase. [EC: 3.5.1.25]

Predicted SEED Role

"N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)" in subsystem Chitin and N-acetylglucosamine utilization or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 3.5.1.25)

MetaCyc Pathways

• N-acetylglucosamine degradation II (3/3 steps found)
• N-acetylglucosamine degradation I (2/2 steps found)
• N-acetyl-D-galactosamine degradation (4/5 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• D-galactosamine and N-acetyl-D-galactosamine degradation (3/4 steps found)
• peptidoglycan recycling I (9/14 steps found)
• superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation (2/6 steps found)
• chitin derivatives degradation (3/8 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.25

Use Curated BLAST to search for 3.5.1.25

Sequence Analysis Tools

See A0KZW7 at UniProt or InterPro

Protein Sequence (378 amino acids)

>Shewana3_3112 N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25) (Shewanella sp. ANA-3)
MKFTLIAEQLFDGEAFHRDVPVTIEDGLIASLDTASGAKEIRYPGTLVPGFIDVQVNGGG
GALFNSSPTVACIETIGKAHARFGTTGFLPTLITDDVQVMAKAADAVASAVAQKSAGVLG
VHFEGPHLSVPKKGVHPQGFIREITEAELAIFCRQDLGIRVVTLAPENVSPEVIRLLVES
GVKVCLGHSNADYDTVVAALAAGATGFTHLYNAMSPLGSREPGVVGAAIESETAWCGLIV
DGHHVHPAAARVALRAKPRGKVMLVTDAMPPVGMDDETSFELFGTQVLRVGDRLNAVTGE
LAGCVLDMATAVENSVKMLGLPLGEALRMASLYPAEFLGIAESVGRLAVGQRADLVLLDN
QYKVLANYIAGNAVYVRP