Protein Info for Shewana3_3052 in Shewanella sp. ANA-3

Annotation: methylthioadenosine nucleosidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01048: PNP_UDP_1
  • amino acids 2 to 206 (205 residues), 180.7 bits, E=1.5e-57
• TIGR01704: MTA/SAH nucleosidase
  • amino acids 2 to 229 (228 residues), 275.9 bits, E=1.3e-86

Best Hits

Swiss-Prot: 100% identical to MTNN_SHESA: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K01243, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase [EC: 3.2.2.9] (inferred from 99% identity to shm:Shewmr7_2956)

MetaCyc: 57% identical to 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase monomer (Vibrio cholerae)
Methylthioadenosine nucleosidase. [EC: 3.2.2.16, 3.2.2.9]; 3.2.2.9 [EC: 3.2.2.16, 3.2.2.9]

Predicted SEED Role

"5'-methylthioadenosine nucleosidase (EC 3.2.2.16) @ S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)" (EC 3.2.2.16, EC 3.2.2.9)

MetaCyc Pathways

• S-adenosyl-L-methionine salvage I (4/4 steps found)
• autoinducer AI-2 biosynthesis II (Vibrio) (5/6 steps found)
• autoinducer AI-2 biosynthesis I (4/5 steps found)
• S-methyl-5'-thioadenosine degradation IV (1/1 steps found)
• S-methyl-5'-thioadenosine degradation I (1/2 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (4/7 steps found)
• 5'-deoxyadenosine degradation II (1/4 steps found)
• L-methionine salvage cycle I (bacteria and plants) (6/12 steps found)
• L-methionine salvage cycle II (plants) (4/11 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.2.16 or 3.2.2.9

Sequence Analysis Tools

See A0KZQ7 at UniProt or InterPro

Protein Sequence (236 amino acids)

>Shewana3_3052 methylthioadenosine nucleosidase (RefSeq) (Shewanella sp. ANA-3)
MKIGIIGAMEPEVAHLIAAMTNASSQTIAGIEFIAGTLAGKDVVVTRSGIGKVAASIATT
LLIEKYAPDAVINTGSAGGFADSLSIGDIVISSEVRHHDVDVTAFGYEIGQMAQQPAAFI
PAPYLVEAANKAIAQLGEVKAIEGLICTGDSFICDPVRTKTMLEHFPTMAACEMEGAAIA
QVCHQFGVPFVVIRSLSDNANNDSPVDFDSYIVKAGYHSALMVMLLLEQLDPNSVK