Protein Info for Shewana3_2961 in Shewanella sp. ANA-3

Annotation: N-6 DNA methylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 530 PF12161: HsdM_N" amino acids 7 to 86 (80 residues), 35.3 bits, see alignment E=1.8e-12 PF02384: N6_Mtase" amino acids 142 to 462 (321 residues), 268.4 bits, see alignment E=9.4e-84

Best Hits

Swiss-Prot: 61% identical to T1M_SALTY: Type I restriction enzyme StySJI M protein (hsdM) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 100% identity to shn:Shewana3_2961)

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KZG8 at UniProt or InterPro

Protein Sequence (530 amino acids)

>Shewana3_2961 N-6 DNA methylase (RefSeq) (Shewanella sp. ANA-3)
MTQNDIVQKLWNLCDILRDDGINYSDYVTELVLLLFIKMVHENTEAELLDKHTLPEGYRW
TDLHTKSGINLLNDYKALLLALSTGKRVMLDPNDPEKTIEVQVHNDPLISAIYADAQTRL
REPRHLEQITKTLDQIDWFSAKRDGLGDLYEGLLEKNASETKSGAGQYFTPRVLINSMVR
CIKPQAGEYIQDPAAGTAGFLIAAHEYIKAQPEYDDLSLKQIDFQRYHAYVGVELVPNTR
RLALMNCLLHGMEGDDDGVVHLGNALGNVGQSLKPADVILANPPFGTSKGGEASITRDDL
TFDTSNKQLAFLQHIYRNLKPGGRAAVVLPDNVLFEAGKGTDIRRDLMDKCNLHTILRLP
TGIFYAQGVKTNVLFFTKGSAKDKHQQEQCTENVWVYDLRTNMPSFGKRTPFSDNHLAPF
EQVYGEHAGGLSPRTEGEYSFGAEQVEIADTDENQGVDNRLVHSRWRCFSRDWIRDHKGD
SLDISWLKDKDSVDAASLPEPDVLAREAKAELSAALNELDALLKALGGAV