Protein Info for Shewana3_2770 in Shewanella sp. ANA-3

Annotation: methylmalonate-semialdehyde dehydrogenase [acylating] (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 74% identical to MMSA_PSEAE: Methylmalonate-semialdehyde dehydrogenase [acylating] (mmsA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 99% identity to shm:Shewmr7_2663)

MetaCyc: 74% identical to methylmalonate-semialdehyde dehydrogenase subunit (Pseudomonas aeruginosa)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)

MetaCyc Pathways

β-alanine degradation II (2/2 steps found)
L-valine degradation I (6/8 steps found)
β-alanine degradation I (1/2 steps found)
propanoyl-CoA degradation II (3/5 steps found)
2,4-dinitrotoluene degradation (1/7 steps found)
myo-inositol degradation I (1/7 steps found)
myo-, chiro- and scyllo-inositol degradation (1/10 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.27

Use Curated BLAST to search for 1.2.1.27

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KYX8 at UniProt or InterPro

Protein Sequence (499 amino acids)

>Shewana3_2770 methylmalonate-semialdehyde dehydrogenase [acylating] (RefSeq) (Shewanella sp. ANA-3)
MTTQVKHYIDGEFTTGTGTSQIVVTNPANNEPIALINSATADEVHAAIASAKAAFKTWKE
VPVSERARVMLRYQHLLKEHHDELATILAKETGKTFEDAKGDVWRGIEVAEHACNIASLL
MGETVENVARSIDTYSYTQPLGVCAGITPFNFPAMIPLWMFPLAIACGNTFILKPSEQDP
MTPQRLVELFVEAGAPKGVLQLVHGDKTAVDILLADPAVKAISFVGSVAVGQYIYKTGTD
NLKRVQAFAGAKNHCVIMPDANKQQVINNLVGASVGAAGQRCMAISVAVFVGAAKEWIPE
LKEALAKVRPGLWDDKEAGYGPLISPAAKARVLKLIAQGKEEGAECLLDGSDFSVAGYES
GNWVGPTMFTKVTTDMSIYKEEIFGPVLCCMESDSLEEAIELVNASPYGNGTSIFTASGA
AARKYQHEIEVGQVGINVPIPVPLPFFSFTGWKGSFYGDQHAYGKQAVRFYTETKTITSR
WFESDIAVAAGPNMSINLR