Protein Info for Shewana3_2712 in Shewanella sp. ANA-3

Annotation: 3-beta hydroxysteroid dehydrogenase/isomerase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 394 PF04321: RmlD_sub_bind" amino acids 64 to 215 (152 residues), 34.1 bits, see alignment E=6.7e-12 PF01370: Epimerase" amino acids 64 to 281 (218 residues), 104 bits, see alignment E=3.5e-33 PF16363: GDP_Man_Dehyd" amino acids 64 to 213 (150 residues), 38 bits, see alignment E=5.5e-13 PF05368: NmrA" amino acids 65 to 172 (108 residues), 24.6 bits, see alignment E=6.6e-09 PF01073: 3Beta_HSD" amino acids 65 to 313 (249 residues), 186.1 bits, see alignment E=3e-58 PF02719: Polysacc_synt_2" amino acids 65 to 170 (106 residues), 21.8 bits, see alignment E=3.8e-08 PF13460: NAD_binding_10" amino acids 67 to 207 (141 residues), 58.9 bits, see alignment E=2.6e-19 PF07993: NAD_binding_4" amino acids 113 to 238 (126 residues), 37.7 bits, see alignment E=5.4e-13

Best Hits

Swiss-Prot: 85% identical to OLED_SHEON: 2-alkyl-3-oxoalkanoate reductase (oleD) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: None (inferred from 100% identity to shn:Shewana3_2712)

MetaCyc: 85% identical to hentriaconta-3,6,9,12,19,22,25,28-octaene-16-one-15-oate reductase (Shewanella oneidensis MR-1)
RXN-18561 [EC: 1.1.1.412]

Predicted SEED Role

"NAD(P)H steroid dehydrogenase-like protein in alkane synthesis cluster"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.412

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KYS0 at UniProt or InterPro

Protein Sequence (394 amino acids)

>Shewana3_2712 3-beta hydroxysteroid dehydrogenase/isomerase (RefSeq) (Shewanella sp. ANA-3)
MHLITELNTKMTLTVTECDMTDNPVVLQADTTPTGRPDNLAQAGFDPREQAALYALAAKV
SHAFVTGAGGFLGKAICLRLVAAGIKVTGFARGHYPELEALGVTMVQGDLAYPEQVKQAM
QGCDIVFHVASKAGVWGDRDSYFCPNVKGAANVIAACKAFKISKLVYTSTPSVTFAGEDE
SGIDESTPYASRFLNYYAHSKAIAEKMMLDANQASITASAYALKTVALRPHLIWGPNDPH
LVPRVLARGRLGKLKLVGREDKLVDTIYIDNAAYAHILAAVELCQVSPKCQGKAYFVSND
EPVTMAKMLNLILACDGLPPVTARVPQTLAYVAGAVLETAYRLLNKQEEPIMTRFVAKQL
SCSHYFDISAAKRDFGYHALVSIEEGMKRLKASL