Protein Info for Shewana3_2531 in Shewanella sp. ANA-3

Annotation: TolB domain-containing protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 442 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR02800: Tol-Pal system beta propeller repeat protein TolB" amino acids 14 to 439 (426 residues), 550.7 bits, see alignment E=1.1e-169 PF04052: TolB_N" amino acids 23 to 127 (105 residues), 98.8 bits, see alignment E=3.8e-32 PF07676: PD40" amino acids 207 to 240 (34 residues), 35.2 bits, see alignment 1.9e-12 amino acids 249 to 284 (36 residues), 40.4 bits, see alignment 4.3e-14 amino acids 293 to 328 (36 residues), 51.9 bits, see alignment 1e-17 amino acids 337 to 370 (34 residues), 15 bits, see alignment 3.9e-06

Best Hits

Swiss-Prot: 100% identical to TOLB_SHESA: Tol-Pal system protein TolB (tolB) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K03641, TolB protein (inferred from 100% identity to shm:Shewmr7_2440)

Predicted SEED Role

"tolB protein precursor, periplasmic protein involved in the tonb-independent uptake of group A colicins" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KY89 at UniProt or InterPro

Protein Sequence (442 amino acids)

>Shewana3_2531 TolB domain-containing protein (RefSeq) (Shewanella sp. ANA-3)
MKILAKWLALAVLLCTMPAKAALDIVITEGVDAARPIAVMPFQWQGAGAPPQAIADVVMS
DLIRSGTFKPLDELGLPQRGIGALAQFDASAWANVGAEAVVVGSVKPYGTDQFLVSFDLI
DLVKAQNQALKGPTSATEFLMDSRQTVISAAQFRQYGHRISDIVYEKLTGIRGAFLTRIS
YVVVNHTQKAAYSLMIADYDGYNEQMLLRSPEPLMSPSWSPDGRRLAYVSFENKKAEIFV
QDLYTQVRTKVSSFPGINGAPTFSPDGKSLAVTLSKDGQPEIYVIDIATKAAKRITNHYS
IDTEPSWYPDGKSLLFTSERGGRPQLYRVDLNSGKVTRETFEGEWNLGGSITPDGRSMIF
VNRTNGKFNIARMDLSTRFMQVLTSTRLDESPSVAPNGTMVIYGTTYQGKQVLAAVSTDG
RFKARLPVGQGEVKSPSWSPFL