Protein Info for Shewana3_2160 in Shewanella sp. ANA-3

Annotation: diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 PF01590: GAF" amino acids 25 to 164 (140 residues), 37.3 bits, see alignment E=9.8e-13 TIGR00229: PAS domain S-box protein" amino acids 173 to 297 (125 residues), 44.5 bits, see alignment E=1.6e-15 PF00989: PAS" amino acids 185 to 282 (98 residues), 27.3 bits, see alignment E=7.8e-10 PF13426: PAS_9" amino acids 190 to 281 (92 residues), 44.3 bits, see alignment E=4.6e-15 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 298 to 454 (157 residues), 62.9 bits, see alignment E=2.9e-21 PF00990: GGDEF" amino acids 306 to 449 (144 residues), 67.8 bits, see alignment E=2.5e-22 PF00563: EAL" amino acids 472 to 705 (234 residues), 235.6 bits, see alignment E=1.4e-73

Best Hits

KEGG orthology group: None (inferred from 100% identity to shn:Shewana3_2160)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KX71 at UniProt or InterPro

Protein Sequence (727 amino acids)

>Shewana3_2160 diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq) (Shewanella sp. ANA-3)
MKRRQHQHLLESLVHSTAKQQGDFTKTAELATELLCQNLAVSLVSVWIFSADQSSQSLVA
QFGAMPLQDTYRPSQLQTLPCYLNELKNHRHIDAGNTLIDPRLTELVDNYFIPRQILSSL
EIGIRINGHLEGVLCLERAQVTHHWHDSEIHLACQMADQLALTLATKHTYDKEERLSLFR
CATEQSRQMSMLINLHTEKVEYVNQAHEEITGLAREKSIGANLRELSFFKQHNQLAEQTL
AQIFRGEQAKGEVQFNRADGSRYWLKYVVSQFITDRGNHYALVSGEESTDEHNYKAELER
LAWRCSLTGLNNRSHFNRVLERTTNGLLLLVDLVAFKRFNDTYGHENGDSLLIEIGRRLK
HFSEINKATEIARVGSDEFAVLLTDDNAVYDLDYFSTRLYQHLAMPILIGREQIEPKPAL
AVVDIASVANLFAPLTCADIAVQYAKKKKGTAIQVFNSTLLSAFKEDAQIERDLHSAIRG
RQFELYYQPLRDLEQKTYIGAEALIRWHHPKKGVLYPGAFIDIAEQSGMINAIGSWVLEA
ACRQLNIWQHHNADLSMHVNVSARQFFSGNLYEQVWQLLTRYRLKPKTLILEITETELMG
DIRHATLLCQELAELGVGLAIDDFGTGYSSMRYLKQFPISKLKIDRSFISDLAISRESQE
IVSAIIAMASALNISLTAEGVETAEQEAFLAKSFCHQAQGYLYSPALREPEFAQFSLVAK
EPNLVTH