Protein Info for Shewana3_1965 in Shewanella sp. ANA-3

Annotation: 2,4-dienoyl-CoA reductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 672 PF00724: Oxidored_FMN" amino acids 6 to 328 (323 residues), 283.7 bits, see alignment E=3.5e-88 PF07992: Pyr_redox_2" amino acids 376 to 654 (279 residues), 84.4 bits, see alignment E=1.4e-27 PF13450: NAD_binding_8" amino acids 379 to 427 (49 residues), 28 bits, see alignment 3.2e-10

Best Hits

Swiss-Prot: 56% identical to FADH_ECOLI: 2,4-dienoyl-CoA reductase (fadH) from Escherichia coli (strain K12)

KEGG orthology group: K00219, 2,4-dienoyl-CoA reductase (NADPH2) [EC: 1.3.1.34] (inferred from 100% identity to shn:Shewana3_1965)

MetaCyc: 56% identical to 2,4-dienoyl-CoA reductase (Escherichia coli K-12 substr. MG1655)
2,4-dienoyl-CoA reductase (NADPH). [EC: 1.3.1.34]

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KWM7 at UniProt or InterPro

Protein Sequence (672 amino acids)

>Shewana3_1965 2,4-dienoyl-CoA reductase (RefSeq) (Shewanella sp. ANA-3)
MSFPHLLEPLDLGFTQLKNRVLMGSMHTGLEEEKGGFEKLAAFYKERALGGVGLIVTGGI
SPNLRGRLTPHACQLSFPWQVGKHRIVTQAVHEAGGKICMQILHAGRYGYHPFSQAPSRI
KSPITPFTPSAMSSRQVRATIKDYASSAALAKRAGYDGVEVMGSEGYLINQFISSRTNTR
TDEWGGSFEKRAQFPIEIVNAIRAKVGKDFIIIFRLSMLDLVDNGSTWDEVVQLAKWLEH
VGVSIINTGIGWHEARVPTIATSVPRGAFAWVTEKLKKSVSVPLIATNRINTPEIGEQII
ASGQADMVSMARPFLADPEFVNKAAANTPELINTCIGCNQACLDHTFSLKRATCLVNPRA
CYETEINFLPTNNKKRIAVMGAGPAGMAFSVYAAMRGHEVVLFEAKSEVGGQFNLARKIP
GKEEFNETIRYFLNQIKLHKIDLRLNTRLDAKVLETETFDEIVIASGVVPRELSLPGFDS
PKVVDYQQVLTGQATIGNKVALIGAGGIGFDMAHYLCESESSTLNPEKWLKQWGIDKQYQ
HAGGLAEPEVDNSDHRQVYLLQRKTSKMGQGLGKTTGWIHRLVLKQHDVKMKTGVNYDKF
DNAGLHIRVGEQTEVLAVDNVILCAGQESNRTLVDEMKATGIPVHLIGGVDVAAELDAKR
AIRQGAELAMRL