Protein Info for Shewana3_1951 in Shewanella sp. ANA-3

Annotation: putative methyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00740: tRNA (cmo5U34)-methyltransferase
  • amino acids 14 to 252 (239 residues), 347.3 bits, E=2.4e-108
• PF01209: Ubie_methyltran
  • amino acids 64 to 175 (112 residues), 23.7 bits, E=1e-08
• PF01135: PCMT
  • amino acids 64 to 135 (72 residues), 20.4 bits, E=1.3e-07
• PF13847: Methyltransf_31
  • amino acids 65 to 176 (112 residues), 34.6 bits, E=5.4e-12
• PF00891: Methyltransf_2
  • amino acids 66 to 185 (120 residues), 22.4 bits, E=2.4e-08
• PF13649: Methyltransf_25
  • amino acids 70 to 168 (99 residues), 58.2 bits, E=3.8e-19
• PF08241: Methyltransf_11
  • amino acids 72 to 172 (101 residues), 45.2 bits, E=4.4e-15
• PF08242: Methyltransf_12
  • amino acids 72 to 170 (99 residues), 42 bits, E=4.4e-14

Best Hits

Swiss-Prot: 100% identical to CMOA_SHESA: Carboxy-S-adenosyl-L-methionine synthase (cmoA) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K15256, tRNA (cmo5U34)-methyltransferase [EC: 2.1.1.-] (inferred from 100% identity to shn:Shewana3_1951)

MetaCyc: 62% identical to carboxy-S-adenosyl-L-methionine synthase (Escherichia coli K-12 substr. MG1655)
RXN0-7066

Predicted SEED Role

"tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase"

MetaCyc Pathways

• 5-(methoxycarbonylmethoxy)uridine biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

See A0KWL3 at UniProt or InterPro

Protein Sequence (252 amino acids)

>Shewana3_1951 putative methyltransferase (RefSeq) (Shewanella sp. ANA-3)
MAEFSRYSQMNASQDTIYAQASEHISDFQFDNRVAGVFSDMIRRSVPGYTQIINTIGDFA
DRFVKPNTQVYDLGCSLGAATLSIRRQIQGRDCRIIAVDNSESMVTRCQENLNAYVSDTE
VELICGDIRDIHIENASLVVLNFTLQFLPPEDREALIAKIYHGLNPGGLLVLSEKIRFDD
APIQSVLEELHLDFKRANGYSELEISQKRSALENVMKPDTLTLHQQRLTRQGFSHFSLWF
QCFNFSSMVAIK